The saccharomyces PIF1 DNA helicase inhibits telomere elongation and de novo telomere formation

Vincent P. Schulz, Virginia Araxie Zakian

Research output: Contribution to journalArticle

278 Scopus citations

Abstract

A screen to detect yeast mutants that frequently lost expression of subtelomeric genes identified two mutations in PIF1, a gene known to encode a 5′ to 3′ DNA helicase. The loss of expression of subtelomeric genes in pif1 cells was due to deletion of the subtelomeric regions of the chromosomes and the generation of new telomeres at proximal sites. In pif1 mutants, de novo telomere formation usually occurred at sites with very little homology to telomeric DNA. De novo telomere formation after HO-induced chromosome breakage also occurred at elevated frequencies in pif1 cells. Moreover, mutations in PIF1 caused all telomeres to lengthen. These results suggest that the PIF1 helicase is an inhibitor of both de novo telomere formation and telomere elongation.

Original languageEnglish (US)
Pages (from-to)145-155
Number of pages11
JournalCell
Volume76
Issue number1
DOIs
StatePublished - Jan 14 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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