TY - JOUR
T1 - The rudimentary locus of Drosophila melanogaster
AU - Segraves, William A.
AU - Louis, Christos
AU - Tsubota, Stuart
AU - Schedl, Paul
AU - Rawls, John M.
AU - Jarry, Bruno P.
N1 - Funding Information:
We thank G. Stark and members of his group (W. Liao, R. Padgett and K. Shigesada) for communicating their results and making their cDNA clones available prior to this publication, and M. Goldschmidt-Clermont for helpful discussions and the gift of his Drosophila cDNA banks. We also thank Drs A. Udvardy and T. Woodrow Cline for stimulating discussions and Christian Schatz for his assistance in the electron microscopy. This work was supported by grants GM 25976 from the National Institutes of Health. CD 42B from t,he American Cancer Society and l-830 from the March of Dimes (top.&), grants PCM 78-14164 and AM 29738 from the National Science Foundation and the National Tnstitutes of Health, as well as a Research Career Development Award (AM00676: to J.M.R.) and a grant from the Centre National de la Recherche Scientifique (ATP0065051: to B.P.,J.).
PY - 1984/5/5
Y1 - 1984/5/5
N2 - In the studies reported here, we have examined the molecular organization of the rudimentary gene of Drosophila melanogaster. rudimentary encodes a 220,000 Mr polypeptide catalyzing the first three steps in pyrimidine biosynthesis: carbamyl phosphate synthetase, aspartate transcarbamylase and dihydroorotase. We have determined the direction of transcription of the gene relative to the genetic map of the locus. The 5′ end maps to the distal end of the locus (relative to the centromere) and transcription proceeds through the domains encoding dihydroorotase, carbamyl phosphate synthetase and finally aspartate transcarbamylase. The rudimentary transcription unit spans a DNA segment of 13.2 × 103 base-pairs and encodes a mature messenger RNA of 7.3 × 103 base-pairs. Three intervening sequences have been identified, one of which is over 4 × 103 base-pairs in length. Finally, we have compared the DNA sequence organization of the Drosophila rudimentary gene with the corresponding loci of yeast and hamster.
AB - In the studies reported here, we have examined the molecular organization of the rudimentary gene of Drosophila melanogaster. rudimentary encodes a 220,000 Mr polypeptide catalyzing the first three steps in pyrimidine biosynthesis: carbamyl phosphate synthetase, aspartate transcarbamylase and dihydroorotase. We have determined the direction of transcription of the gene relative to the genetic map of the locus. The 5′ end maps to the distal end of the locus (relative to the centromere) and transcription proceeds through the domains encoding dihydroorotase, carbamyl phosphate synthetase and finally aspartate transcarbamylase. The rudimentary transcription unit spans a DNA segment of 13.2 × 103 base-pairs and encodes a mature messenger RNA of 7.3 × 103 base-pairs. Three intervening sequences have been identified, one of which is over 4 × 103 base-pairs in length. Finally, we have compared the DNA sequence organization of the Drosophila rudimentary gene with the corresponding loci of yeast and hamster.
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U2 - 10.1016/0022-2836(84)90441-8
DO - 10.1016/0022-2836(84)90441-8
M3 - Article
C2 - 6144799
AN - SCOPUS:0021612041
VL - 175
SP - 1
EP - 17
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 1
ER -