The response regulator SprE controls the stability of RpoS

Leslie A. Pratt, Thomas J. Silhavy

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212 Scopus citations


In Escherichia coli, the sigma factor, RpoS, is a central regulator in stationary-phase cells. We have identified a gene, sprE (stationary-phase regulator), as essential for the negative regulation of rpoS expression. SprE negatively regulates the rpoS gene product at the level of protein stability, perhaps in response to nutrient availability. The ability of SprE to destabilize RpoS is dependent on the ClpX/ClpP protease. Based on homology, SprE is a member of the response regulator family of proteins. SprE is the first response regulator identified that is implicated in the control of protein stability. Moreover, SprE is the first reported protein that appears to regulate rpoS in response to a specific environmental parameter.

Original languageEnglish (US)
Pages (from-to)2488-2492
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number6
StatePublished - Mar 19 1996

All Science Journal Classification (ASJC) codes

  • General


  • ClpP
  • ClpX
  • proteolysis
  • sigma factor
  • stationary phase


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