TY - CHAP
T1 - The Regulation of Histidine Sensor Kinase Complexes by Quorum Sensing Signal Molecules
AU - Neiditch, Matthew B.
AU - Hughson, Frederick M.
N1 - Funding Information:
We are immensely grateful to the Princeton colleagues who have participated in our studies of quorum sensing, including Bonnie Bassler, Mike Federle, Danielle Swem, Shawn Campagna, Xin Chen, Phil Jeffrey, Bob Kelly, Stephen Miller, Audra Pompeani, Stephan Schauder, Martin Semmelhack, Michi Taga, and Karina Xavier. Funding for our work in this area has been provided by the National Institutes of Health (AI‐054442 and a postdoctoral fellowship to M. B. N.).
PY - 2007
Y1 - 2007
N2 - Two-component sensor kinase signaling systems are widespread in bacteria, but gaining mechanistic insight into how kinase activity is controlled by ligand binding has proved challenging. Here, we discuss this problem in the context of our structural and functional studies of bacterial quorum sensing receptors. Specifically, this chapter focuses on the transmembrane sensor kinase complex LuxPQ, which serves as the receptor for the "universal" quorum sensing signal molecule autoinducer-2 (AI-2). Methods are presented for the overproduction, purification, crystallization, and functional characterization of LuxPQ's ligand-binding (periplasmic) domain.
AB - Two-component sensor kinase signaling systems are widespread in bacteria, but gaining mechanistic insight into how kinase activity is controlled by ligand binding has proved challenging. Here, we discuss this problem in the context of our structural and functional studies of bacterial quorum sensing receptors. Specifically, this chapter focuses on the transmembrane sensor kinase complex LuxPQ, which serves as the receptor for the "universal" quorum sensing signal molecule autoinducer-2 (AI-2). Methods are presented for the overproduction, purification, crystallization, and functional characterization of LuxPQ's ligand-binding (periplasmic) domain.
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U2 - 10.1016/S0076-6879(07)23011-3
DO - 10.1016/S0076-6879(07)23011-3
M3 - Chapter
C2 - 17609135
AN - SCOPUS:34250899895
T3 - Methods in Enzymology
SP - 250
EP - 263
BT - Two Component Signaling Systems, Part B
PB - Academic Press Inc.
ER -