The red plaque test: A rapid method for identification of excision defective variants of bacteriophage lambda

Lynn William Enquist, R. A. Weisberg

Research output: Contribution to journalArticle

71 Scopus citations

Abstract

We have developed a simple and rapid plaque test for isolation, characterization, and mapping of integration and excision-defective mutants of bacteriophage lambda. Mutants are recognized by their inability to promote the int and xis dependent excision of a cryptic prophage inserted within the galT gene of E. coli. Methods for complementation of int and xis mutants as well as methods for isolation and analysis of transducing phage are presented.

Original languageEnglish (US)
Pages (from-to)147-153
Number of pages7
JournalVirology
Volume72
Issue number1
DOIs
StatePublished - Jul 1 1976
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Virology

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