The red plaque test: A rapid method for identification of excision defective variants of bacteriophage lambda

L. W. Enquist; R. A. Weisberg

doi:10.1016/0042-6822(76)90319-6

The red plaque test: A rapid method for identification of excision defective variants of bacteriophage lambda

L. W. Enquist, R. A. Weisberg

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Abstract

We have developed a simple and rapid plaque test for isolation, characterization, and mapping of integration and excision-defective mutants of bacteriophage lambda. Mutants are recognized by their inability to promote the int and xis dependent excision of a cryptic prophage inserted within the galT gene of E. coli. Methods for complementation of int and xis mutants as well as methods for isolation and analysis of transducing phage are presented.

Original language	English (US)
Pages (from-to)	147-153
Number of pages	7
Journal	Virology
Volume	72
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(76)90319-6
State	Published - Jul 1 1976

All Science Journal Classification (ASJC) codes

Virology

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10.1016/0042-6822(76)90319-6

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abstract = "We have developed a simple and rapid plaque test for isolation, characterization, and mapping of integration and excision-defective mutants of bacteriophage lambda. Mutants are recognized by their inability to promote the int and xis dependent excision of a cryptic prophage inserted within the galT gene of E. coli. Methods for complementation of int and xis mutants as well as methods for isolation and analysis of transducing phage are presented.",

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AU - Weisberg, R. A.

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N2 - We have developed a simple and rapid plaque test for isolation, characterization, and mapping of integration and excision-defective mutants of bacteriophage lambda. Mutants are recognized by their inability to promote the int and xis dependent excision of a cryptic prophage inserted within the galT gene of E. coli. Methods for complementation of int and xis mutants as well as methods for isolation and analysis of transducing phage are presented.

AB - We have developed a simple and rapid plaque test for isolation, characterization, and mapping of integration and excision-defective mutants of bacteriophage lambda. Mutants are recognized by their inability to promote the int and xis dependent excision of a cryptic prophage inserted within the galT gene of E. coli. Methods for complementation of int and xis mutants as well as methods for isolation and analysis of transducing phage are presented.

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The red plaque test: A rapid method for identification of excision defective variants of bacteriophage lambda

Abstract

All Science Journal Classification (ASJC) codes

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