The recombinant expression systems for structure determination of eukaryotic membrane proteins

Yuan He, Kan Wang, Nieng Yan

Research output: Contribution to journalReview article

48 Scopus citations

Abstract

Eukaryotic membrane proteins, many of which are key players in various biological processes, constitute more than half of the drug targets and represent important candidates for structural studies. In contrast to their physiological significance, only very limited number of eukaryotic membrane protein structures have been obtained due to the technical challenges in the generation of recombinant proteins. In this review, we examine the major recombinant expression systems for eukaryotic membrane proteins and compare their relative advantages and disadvantages. We also attempted to summarize the recent technical strategies in the advancement of eukaryotic membrane protein purification and crystallization.

Original languageEnglish (US)
Pages (from-to)658-672
Number of pages15
JournalProtein & cell
Volume5
Issue number9
DOIs
StatePublished - Sep 1 2014

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Drug Discovery
  • Cell Biology

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