TY - JOUR
T1 - The pyrenoid
T2 - The eukaryotic CO2-concentrating organelle
AU - He, Shan
AU - Crans, Victoria L.
AU - Jonikas, Martin C.
N1 - Funding Information:
We thank Alistair McCormick (University of Edinburgh), Moritz Meyer (Princeton alumni), Ned Wingreen (Princeton University), Debashish Bhattacharya (Rutgers University), Ben Engel (University of Basel), Lianyong Wang (Princeton University), Micah Burton (Princeton University), and the two anonymous reviewers for helpful comments, suggestions, and clarifications. We thank Marie Bao, as part of Life Science Editors, for help with manuscript editing. This work was supported by Howard Hughes Medical Institute, National Science Foundation (MCB-1935444), and National Institutes of Health (R01GM140032) grants to M.C.J. M.C.J. is a Howard Hughes Medical Institute Investigator. We apologize to all colleagues whose work we could not incorporate due to space constraints.
Publisher Copyright:
© 2023 The Author(s). Published by Oxford University Press on behalf of American Society of Plant Biologists.
PY - 2023/9
Y1 - 2023/9
N2 - The pyrenoid is a phase-separated organelle that enhances photosynthetic carbon assimilation in most eukaryotic algae and the land plant hornwort lineage. Pyrenoids mediate approximately one-Third of global CO2 fixation, and engineering a pyrenoid into C3 crops is predicted to boost CO2 uptake and increase yields. Pyrenoids enhance the activity of the CO2-fixing enzyme Rubisco by supplying it with concentrated CO2. All pyrenoids have a dense matrix of Rubisco associated with photosynthetic thylakoid membranes that are thought to supply concentrated CO2. Many pyrenoids are also surrounded by polysaccharide structures that may slow CO2 leakage. Phylogenetic analysis and pyrenoid morphological diversity support a convergent evolutionary origin for pyrenoids. Most of the molecular understanding of pyrenoids comes from the model green alga Chlamydomonas (Chlamydomonas reinhardtii). The Chlamydomonas pyrenoid exhibits multiple liquid-like behaviors, including internal mixing, division by fission, and dissolution and condensation in response to environmental cues and during the cell cycle. Pyrenoid assembly and function are induced by CO2 availability and light, and although transcriptional regulators have been identified, posttranslational regulation remains to be characterized. Here, we summarize the current knowledge of pyrenoid function, structure, components, and dynamic regulation in Chlamydomonas and extrapolate to pyrenoids in other species.
AB - The pyrenoid is a phase-separated organelle that enhances photosynthetic carbon assimilation in most eukaryotic algae and the land plant hornwort lineage. Pyrenoids mediate approximately one-Third of global CO2 fixation, and engineering a pyrenoid into C3 crops is predicted to boost CO2 uptake and increase yields. Pyrenoids enhance the activity of the CO2-fixing enzyme Rubisco by supplying it with concentrated CO2. All pyrenoids have a dense matrix of Rubisco associated with photosynthetic thylakoid membranes that are thought to supply concentrated CO2. Many pyrenoids are also surrounded by polysaccharide structures that may slow CO2 leakage. Phylogenetic analysis and pyrenoid morphological diversity support a convergent evolutionary origin for pyrenoids. Most of the molecular understanding of pyrenoids comes from the model green alga Chlamydomonas (Chlamydomonas reinhardtii). The Chlamydomonas pyrenoid exhibits multiple liquid-like behaviors, including internal mixing, division by fission, and dissolution and condensation in response to environmental cues and during the cell cycle. Pyrenoid assembly and function are induced by CO2 availability and light, and although transcriptional regulators have been identified, posttranslational regulation remains to be characterized. Here, we summarize the current knowledge of pyrenoid function, structure, components, and dynamic regulation in Chlamydomonas and extrapolate to pyrenoids in other species.
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U2 - 10.1093/plcell/koad157
DO - 10.1093/plcell/koad157
M3 - Review article
C2 - 37279536
AN - SCOPUS:85169502508
SN - 1040-4651
VL - 35
SP - 3236
EP - 3259
JO - Plant Cell
JF - Plant Cell
IS - 9
ER -