The PSI-U1 snRNP interaction regulates male mating behavior in Drosophila

Qingqing Wang, J. Matthew Taliaferro, Ugne Klibaite, Valérie Hilgers, Joshua W. Shaevitz, Donald C. Rio

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Alternative pre-mRNA splicing (AS) is a critical regulatory mechanism that operates extensively in the nervous system to produce diverse protein isoforms. Fruitless AS isoforms have been shown to influence male courtship behavior, but the underlying mechanisms are unknown. Using genome-wide approaches and quantitative behavioral assays, we show that the P-element somatic inhibitor (PSI) and its interaction with the U1 small nuclear ribonucleoprotein complex (snRNP) control male courtship behavior. PSI mutants lacking the U1 snRNP-interacting domain (PSIAB mutant) exhibit extended but futile mating attempts. The PSI&AB mutant results in significant changes in the AS patterns of ∼1,200 genes in the Drosophila brain, many of which have been implicated in the regulation of male courtship behavior. PSI directly regulates the AS of at least one-third of these transcripts, suggesting that PSI-U1 snRNP interactions coordinate the behavioral network underlying courtship behavior. Importantly, one of these direct targets is fruitless, the master regulator of courtship. Thus, PSI imposes a specific mode of regulatory control within the neuronal circuit controlling courtship, even though it is broadly expressed in the fly nervous system. This study reinforces the importance of AS in the control of gene activity in neurons and integrated neuronal circuits, and provides a surprising link between a pleiotropic pre-mRNA splicing pathway and the precise control of successful male mating behavior.

Original languageEnglish (US)
Pages (from-to)5269-5274
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number19
DOIs
StatePublished - May 10 2016

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • Male courtship behavior
  • PSI|
  • U1 snrnp|alternative pre-mRNA splicing|

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