The onset of collective behavior in social amoebae

Thomas Gregor, Koichi Fujimoto, Noritaka Masaki, Satoshi Sawai

Research output: Contribution to journalArticlepeer-review

200 Scopus citations

Abstract

In the social amoebae Dictyostelium discoideum, periodic synthesis and release of extracellular cyclic adenosine 3',5'-monophosphate (cAMP) guide cell aggregation and commitment to form fruiting bodies. It is unclear whether these oscillations are an intrinsic property of individual cells or if they exist only as a population-level phenomenon. Here, we showed by live-cell imaging of intact cell populations that pulses originate from a discrete location despite constant exchange of cells to and from the region. In a perfusion chamber, both isolated single cells and cell populations switched from quiescence to rhythmic activity depending on the concentration of extracellular cAMP. A quantitative analysis showed that stochastic pulsing of individual cells below the threshold concentration of extracellular cAMP plays a critical role in the onset of collective behavior.

Original languageEnglish (US)
Pages (from-to)1021-1025
Number of pages5
JournalScience
Volume328
Issue number5981
DOIs
StatePublished - May 21 2010

All Science Journal Classification (ASJC) codes

  • General

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