TY - JOUR
T1 - The mouse Fused locus encodes axin, an inhibitor of the Wnt signaling pathway that regulates embryonic axis formation
AU - Zeng, Li
AU - Fagotto, François
AU - Zhang, Tong
AU - Hsu, Wei
AU - Vasicek, Thomas J.
AU - Perry, William L.
AU - Lee, James J.
AU - Tilghman, Shirley M.
AU - Gumbiner, Barry M.
AU - Costantini, Frank
N1 - Funding Information:
Correspondence regarding this paper should be addressed to F. C. (e-mail: [email protected]). We thank Jan Kitajewski for comments on the manuscript. This work was supported by grants from the NIH to F. C. (DK-46934) and B. M. G. (GM37432 and NCI-P30-CA-08784) and a fellowship (W. H.) from the National Kidney Foundation. S. M. T. is an Investigator of the Howard Hughes Medical Institute. B. M. G. is a recipient of a Career Scientist Award from the Irma T. Hirschl Trust.
PY - 1997/7/11
Y1 - 1997/7/11
N2 - Mutations at the mouse Fused locus have pleiotropic developmental effects, including the formation of axial duplications in homozygous embryos. The product of the Fused locus, Axin, displays similarities to RGS (Regulators of G-Protein Signaling) and Dishevelled proteins. Mutant Fused alleles that cause axial duplications disrupt the major mRNA, suggesting that Axin negatively regulates the response to an axis-inducing signal. Injection of Axin mRNA into Xenopus embryos inhibits dorsal axis formation by interfering with signaling through the Wnt pathway. Furthermore, ventral injection of an Axin mRNA lacking the RGS domain induces an ectopic axis, apparently through a dominant-negative mechanism. Thus, Axin is a novel inhibitor of Wnt signaling and regulates an early step in embryonic axis formation in mammals and amphibians.
AB - Mutations at the mouse Fused locus have pleiotropic developmental effects, including the formation of axial duplications in homozygous embryos. The product of the Fused locus, Axin, displays similarities to RGS (Regulators of G-Protein Signaling) and Dishevelled proteins. Mutant Fused alleles that cause axial duplications disrupt the major mRNA, suggesting that Axin negatively regulates the response to an axis-inducing signal. Injection of Axin mRNA into Xenopus embryos inhibits dorsal axis formation by interfering with signaling through the Wnt pathway. Furthermore, ventral injection of an Axin mRNA lacking the RGS domain induces an ectopic axis, apparently through a dominant-negative mechanism. Thus, Axin is a novel inhibitor of Wnt signaling and regulates an early step in embryonic axis formation in mammals and amphibians.
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U2 - 10.1016/S0092-8674(00)80324-4
DO - 10.1016/S0092-8674(00)80324-4
M3 - Article
C2 - 9230313
AN - SCOPUS:0031457978
SN - 0092-8674
VL - 90
SP - 181
EP - 192
JO - Cell
JF - Cell
IS - 1
ER -