The metabolism of small cellular RNA species during productive subgroup C adenovirus infection

Jean K. Smiley, Marjorie A. Young, Catherine C. Bansbach, S. J. Flint

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

During the late phase of subgroup C adenovirus infection, export of cellular mRNA from the nucleus to the cytoplasm isinhibited. In one approach to investigate the mechanism whereby viral late mRNAs are selected for export, we have examined the metabolism of small cellular RNA species transcribed by all three RNA polymerases during the late phase of Ad5 infection. No changes in the quantities of [3H]uridine-labeled 5S rRNA or tRNAs entering the cytoplasm were observed in infected cells. Adenovirus type 5 infection reduced the nuclear and cytoplasmic populations of the newly synthesized, snRNP-associated snRNAs U1, U2, U4, U5, and U6. Transcription of a representative snRNA, U1 RNA, was not inhibited, indicating that the post-transcriptional metabolism of snRNAs was perturbed during the late phase of infection. The increased cytoplasmic concentration of newly synthesized U1 RNA in Ad5- compared to mock-infected cells, and the greater reduction of the snRNP-associated compared to the total U1 RNA population, indicated that snRNP assembly in the cytoplasm was impaired. As adenovirus infection does not perturb export from the nucleus of small cellular mRNAs transcribed by RNA polymerases II and III, viral mRNA must be distinguished for selective export at a nuclear step upstream of translocation to the cytoplasm via nuclear pore complexes.

Original languageEnglish (US)
Article number95800247
Pages (from-to)100-107
Number of pages8
JournalVirology
Volume206
Issue number1
DOIs
StatePublished - Jan 10 1995

All Science Journal Classification (ASJC) codes

  • Virology

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    Smiley, J. K., Young, M. A., Bansbach, C. C., & Flint, S. J. (1995). The metabolism of small cellular RNA species during productive subgroup C adenovirus infection. Virology, 206(1), 100-107. [95800247]. https://doi.org/10.1016/S0042-6822(95)80024-7