The Master Switch Gene Sex-lethal Promotes Female Development by Negatively Regulating the N-Signaling Pathway

Jill K.M. Penn, Paul Schedl

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Notch (N) signaling is used for cell-fate determination in many different developmental contexts. Here, we show that the master control gene for sex determination in Drosophila melanogaster, Sex-lethal (Sxl), negatively regulates the N-signaling pathway in females. In genetic assays, reducing Sxl activity suppresses the phenotypic effects of N mutations, while increasing Sxl activity enhances the effects. Sxl appears to negatively regulate the pathway by reducing N protein accumulation, and higher levels of N are found in Sxl- clones than in adjacent wild-type cells. The inhibition of N expression does not depend on the known downstream targets of Sxl; however, we find that Sxl protein can bind to N mRNAs. Finally, our results indicate that downregulation of the N pathway by Sxl contributes to sex-specific differences in morphology and suggest that it may also play an important role in follicle cell specification during oogenesis.

Original languageEnglish (US)
Pages (from-to)275-286
Number of pages12
JournalDevelopmental cell
Volume12
Issue number2
DOIs
StatePublished - Feb 2007

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

Keywords

  • DEVBIO

Fingerprint Dive into the research topics of 'The Master Switch Gene Sex-lethal Promotes Female Development by Negatively Regulating the N-Signaling Pathway'. Together they form a unique fingerprint.

  • Cite this