The LysR homolog LrhA promotes RpoS degradation by modulating activity of the response regulator SprE

Katherine E. Gibson, Thomas J. Silhavy

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Synthesis of the OmpF porin of Escherichia coli is regulated in response to environmental and growth phase signals. In order to identify constituents of the various regulatory pathways involved in modulating ompF transcriptional expression, transposon insertion mutagenesis was performed and mutations that increased ompF'-lacZ activity were identified as previously described. Mutations mapping to a previously identified gene of unknown function, lrhA, were obtained. We found that LrhA, a LysR homolog, functions as a regulatory component in the RpoS-dependent growth phase repression of ompF. In addition to altered growth phase regulation of ompF, these lrhA mutants have pleiotropic stationary-phase defects as a result of decreased RpoS levels. We provide evidence that LrhA promotes degradation of RpoS by functioning within a genetic pathway that includes the response regulator SprE and the ClpXP protease. LrhA functions upstream of the other components in the pathway and appears to modulate the activity of SprE.

Original languageEnglish (US)
Pages (from-to)563-571
Number of pages9
JournalJournal of bacteriology
Volume181
Issue number2
DOIs
StatePublished - Jan 1999

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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