The lim domain only protein 7 is important in zebrafish heart development

Elisabeth B. Ott, Nynke M.S. Van Den Akker, Philippe A. Sakalis, Adriana C. Gittenberger-de Groot, Aartjan J.W. Te Velthuis, Christoph P. Bagowski

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


The LIM domain only protein 7 (LMO7), a member of the PDZ and LIM domain-containing protein family is a candidate gene with possible roles in embryonic development and breast cancer progression. LMO7 has been linked to actin cytoskeleton organization through nectin/afadin and to cell-cell adhesion by means of E-cadherin/catenin. In addition, LMO7 has been shown to regulate transcription of the nuclear membrane protein Emerin and other muscle relevant genes. In this study, we used in situ hybridization to investigate LMO7 expression during embryonic development in three widely used vertebrate model species: the zebrafish, the chicken and the mouse. Our temporal and spatial gene expression analysis revealed both common and distinct patterns between these species. In mouse and chicken embryos we found expression in the outflow tract, the inflow tract, the pro-epicardial organ and the second heart field, structures highly important in the developing heart. Furthermore, gene knockdown experiments in zebrafish embryos resulted in severe defects in heart development with effects on the conduction system and on heart localization. In summary, we present here the first developmental study of LMO7. We reveal the temporal and spatial expression patterns of this important gene during mouse, chicken and fish development and our findings suggest essential functions for LMO7 during vertebrate heart development.

Original languageEnglish (US)
Pages (from-to)3940-3952
Number of pages13
JournalDevelopmental Dynamics
Issue number12
StatePublished - Dec 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Developmental Biology


  • Calponin homology domain
  • Chicken
  • EDMD
  • Gene expression
  • Heart development
  • In situ hybridization
  • LIM
  • LIM domain only 7
  • LMO7
  • Mouse
  • Notch signaling
  • Notch1b
  • PCD1
  • PDZ
  • X-linked Emery-Dreifuss muscular dystrophy


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