The intervening sequence of a mouse β-globin gene is transcribed within the 15S β-globin mRNA precursor

S. M. Tilghman, P. J. Curtis, D. C. Tiemeier, P. Leder, C. Weissmann

Research output: Contribution to journalArticlepeer-review

165 Scopus citations

Abstract

Mouse β-globin is encoded in a discontinuous structural gene interrupted by a 550-base pair intervening sequence of DNA. Correspondingly, the mature β-globin mRNA appears to be synthesized via a 15S precursor, the length of which roughly equals the total length of the coding and intervening sequences of the β-globin gene. Using the electron microscope to visualize hybrid structures formed between this gene and the purified 15S β-globin mRNA precursor, we show that the intervening sequence is present within the larger precursor molecule. This finding suggests that the precursor mRNA is processed through the removal and rejoining of internal RNA sequences.

Original languageEnglish (US)
Pages (from-to)1309-1313
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume75
Issue number3
DOIs
StatePublished - 1978

All Science Journal Classification (ASJC) codes

  • General

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