TY - JOUR
T1 - The insulator functions of the Drosophila polydactyl C2H2 zinc finger protein CTCF
T2 - Necessity versus sufficiency
AU - Kyrchanova, Olga
AU - Maksimenko, Oksana
AU - Ibragimov, Airat
AU - Sokolov, Vladimir
AU - Postika, Nikolay
AU - Lukyanova, Maria
AU - Schedl, Paul
AU - Georgiev, Pavel
N1 - Funding Information:
This work was supported by the Russian Science Foundation project no. 19-74-30026 (to P.G.). CRISPR-Cas9–directed editing and embryo immunostaining were supported by grant 075-15-2019-1661 from the Ministry of Science and Higher Education of the Russian Federation. Confocal imaging was supported by GM R35GM126975 (to P.S.).
Publisher Copyright:
Copyright © 2020 The Authors, some rights reserved.
PY - 2020
Y1 - 2020
N2 - In mammals, a C2H2 zinc finger (C2H2) protein, CTCF, acts as the master regulator of chromosomal architecture and of the expression of Hox gene clusters. Like mammalian CTCF, the Drosophila homolog, dCTCF, localizes to boundaries in the bithorax complex (BX-C). Here, we have determined the minimal requirements for the assembly of a functional boundary by dCTCF and two other C2H2 zinc finger proteins, Pita and Su(Hw). Although binding sites for these proteins are essential for the insulator activity of BX-C boundaries, these binding sites alone are insufficient to create a functional boundary. dCTCF cannot effectively bind to a single recognition sequence in chromatin or generate a functional insulator without the help of additional proteins. In addition, for boundary elements in BX-C at least four binding sites for dCTCF or the presence of additional DNA binding factors is required to generate a functional insulator.
AB - In mammals, a C2H2 zinc finger (C2H2) protein, CTCF, acts as the master regulator of chromosomal architecture and of the expression of Hox gene clusters. Like mammalian CTCF, the Drosophila homolog, dCTCF, localizes to boundaries in the bithorax complex (BX-C). Here, we have determined the minimal requirements for the assembly of a functional boundary by dCTCF and two other C2H2 zinc finger proteins, Pita and Su(Hw). Although binding sites for these proteins are essential for the insulator activity of BX-C boundaries, these binding sites alone are insufficient to create a functional boundary. dCTCF cannot effectively bind to a single recognition sequence in chromatin or generate a functional insulator without the help of additional proteins. In addition, for boundary elements in BX-C at least four binding sites for dCTCF or the presence of additional DNA binding factors is required to generate a functional insulator.
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U2 - 10.1126/sciadv.aaz3152
DO - 10.1126/sciadv.aaz3152
M3 - Article
C2 - 32232161
AN - SCOPUS:85082491837
SN - 2375-2548
VL - 6
JO - Science Advances
JF - Science Advances
IS - 13
M1 - eaaz3152
ER -