TY - JOUR
T1 - The GAGA factor regulatory network
T2 - Identification of GAGA factor associated proteins
AU - Lomaev, Dmitry
AU - Mikhailova, Anna
AU - Erokhin, Maksim
AU - Shaposhnikov, Alexander V.
AU - Moresco, James J.
AU - Blokhina, Tatiana
AU - Wolle, Daniel
AU - Aoki, Tsutomu
AU - Ryabykh, Vladimir
AU - Yates, John R.
AU - Shidlovskii, Yulii V.
AU - Georgiev, Pavel
AU - Schedl, Paul
AU - Chetverina, Darya
N1 - Publisher Copyright:
© 2017 Lomaev et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2017/3
Y1 - 2017/3
N2 - The Drosophila GAGA factor (GAF) has an extraordinarily diverse set of functions that include the activation and silencing of gene expression, nucleosome organization and remodeling, higher order chromosome architecture and mitosis. One hypothesis that could account for these diverse activities is that GAF is able to interact with partners that have specific and dedicated functions. To test this possibility we used affinity purification coupled with high throughput mass spectrometry to identify GAF associated partners. Consistent with this hypothesis the GAF interacting network includes a large collection of factors and complexes that have been implicated in many different aspects of gene activity, chromosome structure and function. Moreover, we show that GAF interactions with a small subset of partners is direct; however for many others the interactions could be indirect, and depend upon intermediates that serve to diversify the functional capabilities of the GAF protein.
AB - The Drosophila GAGA factor (GAF) has an extraordinarily diverse set of functions that include the activation and silencing of gene expression, nucleosome organization and remodeling, higher order chromosome architecture and mitosis. One hypothesis that could account for these diverse activities is that GAF is able to interact with partners that have specific and dedicated functions. To test this possibility we used affinity purification coupled with high throughput mass spectrometry to identify GAF associated partners. Consistent with this hypothesis the GAF interacting network includes a large collection of factors and complexes that have been implicated in many different aspects of gene activity, chromosome structure and function. Moreover, we show that GAF interactions with a small subset of partners is direct; however for many others the interactions could be indirect, and depend upon intermediates that serve to diversify the functional capabilities of the GAF protein.
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U2 - 10.1371/journal.pone.0173602
DO - 10.1371/journal.pone.0173602
M3 - Article
C2 - 28296955
AN - SCOPUS:85015308895
SN - 1932-6203
VL - 12
JO - PloS one
JF - PloS one
IS - 3
M1 - e0173602
ER -