TY - JOUR
T1 - The effects of membrane cholesterol and simvastatin on red blood cell deformability and ATP release
AU - Forsyth, Alison M.
AU - Braunmüller, Susanne
AU - Wan, Jiandi
AU - Franke, Thomas
AU - Stone, Howard A.
N1 - Funding Information:
T. F. and S. B. acknowledge support of the German Excellence Initiative via NIM , the Centre for Nanoscience CeNS , the DAAD , and the German Research Society DFG via the priority programs SPP1164 and SPP1253 . T. F. and S. B. also thank Achim Wixforth for continuous support.
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/5
Y1 - 2012/5
N2 - It is known that deformation of red blood cells (RBCs) is linked to ATP release from the cells. Further, membrane cholesterol has been shown to alter properties of the cell membrane such as fluidity and bending stiffness. Membrane cholesterol content is increased in some cardiovascular diseases, for example, in individuals with acute coronary syndromes and chronic stable angina, and therefore, because of the potential clinical relevance, we investigated the influence of altered RBC membrane cholesterol levels on ATP release. Because of the correlation between statins and reduced membrane cholesterol in vivo, we also investigated the effects of simvastatin on RBC deformation and ATP release. We found that reducing membrane cholesterol increases cell deformability and ATP release. We also found that simvastatin increases deformability by acting directly on the membrane in the absence of the liver, and that ATP release was increased for cells with enriched cholesterol after treatment with simvastatin.
AB - It is known that deformation of red blood cells (RBCs) is linked to ATP release from the cells. Further, membrane cholesterol has been shown to alter properties of the cell membrane such as fluidity and bending stiffness. Membrane cholesterol content is increased in some cardiovascular diseases, for example, in individuals with acute coronary syndromes and chronic stable angina, and therefore, because of the potential clinical relevance, we investigated the influence of altered RBC membrane cholesterol levels on ATP release. Because of the correlation between statins and reduced membrane cholesterol in vivo, we also investigated the effects of simvastatin on RBC deformation and ATP release. We found that reducing membrane cholesterol increases cell deformability and ATP release. We also found that simvastatin increases deformability by acting directly on the membrane in the absence of the liver, and that ATP release was increased for cells with enriched cholesterol after treatment with simvastatin.
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U2 - 10.1016/j.mvr.2012.02.004
DO - 10.1016/j.mvr.2012.02.004
M3 - Article
C2 - 22349292
AN - SCOPUS:84859509670
SN - 0026-2862
VL - 83
SP - 347
EP - 351
JO - Microvascular Research
JF - Microvascular Research
IS - 3
ER -