The dynamics of genomic-length DNA molecules in 100-nm channels

Jonas O. Tegenfeldt, Christelle Prinz, Han Cao, Steven Chou, Walter W. Reisner, Robert Riehn, Yan Mei Wang, Edward C. Cox, James C. Sturm, Pascal Silberzan, Robert H. Austin

Research output: Contribution to journalArticlepeer-review

453 Scopus citations


We show that genomic-length DNA molecules imaged in nano-channels have an extension along the channel that scales linearly with the contour length of the polymer, in agreement with the scaling arguments developed by de Gennes for self-avoiding confined polymers. This fundamental relationship allows us to measure directly the contour length of single DNA molecules confined in the channels, and the statistical analysis of the dynamics of the polymer in the nanochannel allows us to compute the SD of the mean of the extension. This statistical analysis allows us to measure the extension of λ DNA multimers with a 130-nm SD in 1 min.

Original languageEnglish (US)
Pages (from-to)10979-10983
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number30
StatePublished - Jul 27 2004

All Science Journal Classification (ASJC) codes

  • General


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