The Drosophila TGF-α-like protein Gurken: Expression and cellular localization during Drosophila oogenesis

F. Shira Neuman-Silberberg, Trudi Schüpbach

Research output: Contribution to journalArticlepeer-review

148 Scopus citations

Abstract

The establishment of anterior-posterior and dorsal-ventral polarity of the Drosophila egg and embryo depends on the function of the genes gurken, cornichon and Egfr (Drosophila epidermal growth factor receptor homolog). These genes encode components of a signal transduction pathway that transmits information between the germline cells and the somatic follicle cells of the ovary. gurken encodes a transforming growth factor-α-like protein and is a putative germline ligand of the Egfr present on the follicle cells. In mid-oogenesis the gurken transcript becomes spatially localized to the future dorsal-anterior cortex of the oocyte. To analyze the distribution pattern of Gurken protein we prepared antibodies against Gurken. We describe here the distribution pattern of the Gurken protein in wild-type ovaries and in ovaries from a number of dorsal-ventral patterning mutants. By immunoblotting we detect one major form of the Gurken protein, which likely corresponds to the unprocessed protein.

Original languageEnglish (US)
Pages (from-to)105-113
Number of pages9
JournalMechanisms of Development
Volume59
Issue number2
DOIs
StatePublished - Oct 1996

All Science Journal Classification (ASJC) codes

  • Embryology
  • Developmental Biology

Keywords

  • Confocal
  • Dorsal-ventral axis
  • Drosophila
  • Oogenesis
  • Signal transduction

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