The Drosophila fragile X protein functions as a negative regulator in the orb autoregulatory pathway

Alexandre Costa, Yan Wang, Thomas C. Dockendorff, Hediye Erdjument-Bromage, Paul Tempst, Paul Schedl, Thomas A. Jongens

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

Translational regulation of maternal mRNAs in distinct temporal and spatial patterns underlies many key decisions in developing eggs and embryos. In Drosophila, Orb is responsible for mediating the translational activation of mRNAs localized within the developing oocyte. Orb is a germline-specific RNA binding protein and is one of the founding members of the CPEB family of translational regulators. Here we show that Orb associates with the Drosophila Fragile X Mental Retardation (dFMR1) protein as part of a ribonucleoprotein complex that controls the localized translation of mRNAs in developing egg chambers. One of the key orb regulatory targets is orb mRNA, and this autoregulatory activity is critical for ensuring that Orb protein is expressed at high levels in the oocyte. We show that dFMR1 functions as a negative regulator in the orb autoregulatory circuit, downregulating orb mRNA translation.

Original languageEnglish (US)
Pages (from-to)331-342
Number of pages12
JournalDevelopmental cell
Volume8
Issue number3
DOIs
StatePublished - Mar 1 2005

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'The Drosophila fragile X protein functions as a negative regulator in the orb autoregulatory pathway'. Together they form a unique fingerprint.

  • Cite this

    Costa, A., Wang, Y., Dockendorff, T. C., Erdjument-Bromage, H., Tempst, P., Schedl, P., & Jongens, T. A. (2005). The Drosophila fragile X protein functions as a negative regulator in the orb autoregulatory pathway. Developmental cell, 8(3), 331-342. https://doi.org/10.1016/j.devcel.2005.01.011