The distribution of microbial taxa in the subsurface water of the kalahari shield, South Africa

T. M. Gihring, D. P. Moser, L. H. Lin, M. Davidson, Tullis C. Onstott, L. Morgan, M. Milleson, T. L. Kieft, E. Trimarco, D. L. Balkwill, M. E. Dollhopf

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58 Scopus citations

Abstract

Microbial communities within deep subsurface environments were analyzed by 16S rRNA gene cloning. Clone libraries from 27 borehole fluid, 7 mining-contaminated, and 5 rock samples were compared. Borehole fluids derived from deep fractures were populated by microbial communities with low diversity with an average of 11 and 5 bacterial and archaeal OTUs respectively. Low taxa richness was likely driven by limited biogeochemical reactions available for growth and not extreme parameters such as pH and temperature. Novel taxa of Firmicutes were discovered, commonly found in warm, slightly alkaline, anoxic fracture fluids. Highly divergent lineages of Archaea, unique to South African deep subsurface fracture fluids, are also described. Clone library clustering analyses based on LIBSHUFF phylogenetic relatedness revealed distinct groups of samples corresponding with sample source and geochemistry.

Original languageEnglish (US)
Pages (from-to)415-430
Number of pages16
JournalHandbook of Environmental Chemistry, Volume 5: Water Pollution
Volume23
Issue number6
DOIs
StatePublished - Sep 1 2006

All Science Journal Classification (ASJC) codes

  • Earth and Planetary Sciences (miscellaneous)
  • Microbiology
  • Environmental Science(all)
  • Environmental Chemistry

Keywords

  • 16S rRNA gene
  • Firmicutes
  • Groundwater
  • Microbial ecology
  • Subsurface

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    Gihring, T. M., Moser, D. P., Lin, L. H., Davidson, M., Onstott, T. C., Morgan, L., Milleson, M., Kieft, T. L., Trimarco, E., Balkwill, D. L., & Dollhopf, M. E. (2006). The distribution of microbial taxa in the subsurface water of the kalahari shield, South Africa. Handbook of Environmental Chemistry, Volume 5: Water Pollution, 23(6), 415-430. https://doi.org/10.1080/01490450600875696