The diagnostic substrate bicyclohexane reveals a radical mechanism for bacterial cytochrome P450 in whole cells

Rachel N. Austin; Dayi Deng; Yongying Jiang; Kate Luddy; Jan B. Van Beilen; Paul R. Ortiz De Montellano; John Taylor Groves

doi:10.1002/anie.200603282

The diagnostic substrate bicyclohexane reveals a radical mechanism for bacterial cytochrome P450 in whole cells

Rachel N. Austin
, Dayi Deng
, Yongying Jiang
, Kate Luddy
, Jan B. Van Beilen
, Paul R. Ortiz De Montellano
, John Taylor Groves

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

(Figure Presented) On home ground: The reaction mechanisms of bacterial alkane-oxidizing cytochrome P450s were determined in their native environment using a novel diagnostic substrate probe, bicyclohexane, in whole cells and cell-free extracts (see picture). Purified P450cam also oxidizes bicyclohexane. Clear evidence for a substrate-based radical with a lifetime of 75-250 ps was obtained.

Original language	English (US)
Pages (from-to)	8192-8194
Number of pages	3
Journal	Angewandte Chemie - International Edition
Volume	45
Issue number	48
DOIs	https://doi.org/10.1002/anie.200603282
State	Published - Dec 11 2006

All Science Journal Classification (ASJC) codes

General Chemistry
Catalysis

Keywords

Alkanes
Cytochromes
Oxidation
Radical ions
Reaction mechanisms

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10.1002/anie.200603282

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title = "The diagnostic substrate bicyclohexane reveals a radical mechanism for bacterial cytochrome P450 in whole cells",

abstract = "(Figure Presented) On home ground: The reaction mechanisms of bacterial alkane-oxidizing cytochrome P450s were determined in their native environment using a novel diagnostic substrate probe, bicyclohexane, in whole cells and cell-free extracts (see picture). Purified P450cam also oxidizes bicyclohexane. Clear evidence for a substrate-based radical with a lifetime of 75-250 ps was obtained.",

keywords = "Alkanes, Cytochromes, Oxidation, Radical ions, Reaction mechanisms",

author = "Austin, \{Rachel N.\} and Dayi Deng and Yongying Jiang and Kate Luddy and \{Van Beilen\}, \{Jan B.\} and \{Ortiz De Montellano\}, \{Paul R.\} and Groves, \{John Taylor\}",

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doi = "10.1002/anie.200603282",

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T1 - The diagnostic substrate bicyclohexane reveals a radical mechanism for bacterial cytochrome P450 in whole cells

AU - Austin, Rachel N.

AU - Deng, Dayi

AU - Jiang, Yongying

AU - Luddy, Kate

AU - Van Beilen, Jan B.

AU - Ortiz De Montellano, Paul R.

AU - Groves, John Taylor

PY - 2006/12/11

Y1 - 2006/12/11

N2 - (Figure Presented) On home ground: The reaction mechanisms of bacterial alkane-oxidizing cytochrome P450s were determined in their native environment using a novel diagnostic substrate probe, bicyclohexane, in whole cells and cell-free extracts (see picture). Purified P450cam also oxidizes bicyclohexane. Clear evidence for a substrate-based radical with a lifetime of 75-250 ps was obtained.

AB - (Figure Presented) On home ground: The reaction mechanisms of bacterial alkane-oxidizing cytochrome P450s were determined in their native environment using a novel diagnostic substrate probe, bicyclohexane, in whole cells and cell-free extracts (see picture). Purified P450cam also oxidizes bicyclohexane. Clear evidence for a substrate-based radical with a lifetime of 75-250 ps was obtained.

KW - Alkanes

KW - Cytochromes

KW - Oxidation

KW - Radical ions

KW - Reaction mechanisms

UR - https://www.scopus.com/pages/publications/33845735601

UR - https://www.scopus.com/pages/publications/33845735601#tab=citedBy

U2 - 10.1002/anie.200603282

DO - 10.1002/anie.200603282

M3 - Article

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AN - SCOPUS:33845735601

SN - 1433-7851

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SP - 8192

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JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 48

ER -

The diagnostic substrate bicyclohexane reveals a radical mechanism for bacterial cytochrome P450 in whole cells

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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