The diagnostic substrate bicyclohexane reveals a radical mechanism for bacterial cytochrome P450 in whole cells

Rachel N. Austin, Dayi Deng, Yongying Jiang, Kate Luddy, Jan B. Van Beilen, Paul R. Ortiz De Montellano, John Taylor Groves

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

(Figure Presented) On home ground: The reaction mechanisms of bacterial alkane-oxidizing cytochrome P450s were determined in their native environment using a novel diagnostic substrate probe, bicyclohexane, in whole cells and cell-free extracts (see picture). Purified P450cam also oxidizes bicyclohexane. Clear evidence for a substrate-based radical with a lifetime of 75-250 ps was obtained.

Original languageEnglish (US)
Pages (from-to)8192-8194
Number of pages3
JournalAngewandte Chemie - International Edition
Volume45
Issue number48
DOIs
StatePublished - Dec 11 2006

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

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