@article{1f983e9a0a9849ef90130b2e98afe45d,
title = "The complex effects of ocean acidification on the prominent N2-fixing cyanobacterium Trichodesmium",
abstract = "Acidification of seawater caused by anthropogenic carbon dioxide (CO2) is anticipated to influence the growth of dinitrogen (N2)-fixing phytoplankton, which contribute a large fraction of primary production in the tropical and subtropical ocean. We found that growth and N2-fixation of the ubiquitous cyanobacterium Trichodesmium decreased under acidified conditions, notwithstanding a beneficial effect of high CO2. Acidification resulted in low cytosolic pH and reduced N2-fixation rates despite elevated nitrogenase concentrations. Low cytosolic pH required increased proton pumping across the thylakoid membrane and elevated adenosine triphosphate production. These requirements were not satisfied under field or experimental iron-limiting conditions, which greatly amplified the negative effect of acidification.",
author = "Haizheng Hong and Rong Shen and Futing Zhang and Zuozhu Wen and Siwei Chang and Wenfang Lin and Kranz, {Sven A.} and Luo, {Ya Wei} and Kao, {Shuh Ji} and Morel, {Francois M. M.} and Dalin Shi",
note = "Funding Information: The authors thank B. Hopkinson for helpful discussions and Y. Zhang for technical assistance with the analysis of diazotroph community structure. The authors gratefully acknowledge the captain and crew of the R/V Dongfanghong 2 for their help on the experiments conducted in the northern South China Sea region. This work was supported by the National Key Research and Development Program of China (no. 2016YFA0601203), the National Science Foundation of China (nos. 41222040 and 41376116), the Recruitment Program of Global Youth Experts of China, and a grant from the U.S. NSF to F.M.M.M. The data presented in all figures are available at https://dx.doi.org/10.1594/PANGAEA.874127. Publisher Copyright: {\textcopyright} 2017, American Association for the Advancement of Science. All rights reserved.",
year = "2017",
month = may,
day = "5",
doi = "10.1126/science.aal2981",
language = "English (US)",
volume = "356",
pages = "527--531",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6337",
}