TY - JOUR
T1 - The clam embryo protein cyclin A induces entry into M phase and the resumption of meiosis in Xenopus oocytes
AU - Swenson, Katherine I.
AU - Farrell, Kevin M.
AU - Ruderman, Joan V.
N1 - Funding Information:
and insightful discussions. We thank Joanne Westendorf, David Paul, John Gerhart, Jim Mailer, Nica Borgesi, Nancy Standart, and Jay Hirsh for helpful discussions and suggestions, and Dan Goodenough for his generous scientific hospitality during the latter part of these experiments. This research was supported by National Science Foundation Grant PCM-82-16917 to J. R. and by National Institutes of Health Postdoctoral Fellowship F32 Al 06745-03CIN to K. S.
PY - 1986/12/26
Y1 - 1986/12/26
N2 - Fertilized clam embryos synthesize several new cell-cycle-related proteins. The cloned cDNA and derived amino acid sequences of one of these, cyclin A, are presented here. Immunoblots with an anti-cyclin A antibody reveal that cyclin A is undetectable in oocytes, appears within 15 min of fertilization, and is destroyed near the end of each meiosis and mitosis. We directly tested the ability of cyclin A to induce M phase by injecting SP6 cyclin A mRNA into Xenopus oocytes, which are arrested at the G2/M border of first meiosis. The injected mRNA was translated, with the result that the Xenopus oocytes entered meiosis. These findings indicate that the rise in cyclin A plays a direct and natural role in driving cells into M phase.
AB - Fertilized clam embryos synthesize several new cell-cycle-related proteins. The cloned cDNA and derived amino acid sequences of one of these, cyclin A, are presented here. Immunoblots with an anti-cyclin A antibody reveal that cyclin A is undetectable in oocytes, appears within 15 min of fertilization, and is destroyed near the end of each meiosis and mitosis. We directly tested the ability of cyclin A to induce M phase by injecting SP6 cyclin A mRNA into Xenopus oocytes, which are arrested at the G2/M border of first meiosis. The injected mRNA was translated, with the result that the Xenopus oocytes entered meiosis. These findings indicate that the rise in cyclin A plays a direct and natural role in driving cells into M phase.
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U2 - 10.1016/0092-8674(86)90801-9
DO - 10.1016/0092-8674(86)90801-9
M3 - Article
C2 - 2946420
AN - SCOPUS:0022915860
SN - 0092-8674
VL - 47
SP - 861
EP - 870
JO - Cell
JF - Cell
IS - 6
ER -