The C. elegans microRNA let-7 binds to imperfect let-7 complementary sites from the lin-41 3′UTR

Monica C. Vella, Eun Young Choi, Shin Yi Lin, Kristy Reinert, Frank J. Slack

Research output: Contribution to journalArticle

334 Scopus citations

Abstract

Caenorhabditis elegans let-7, a founding member of the microRNA family, is predicted to bind to six sites in the 3′UTR of the mRNA of its target gene, lin-41, to down-regulate LIN-41. Here, we demonstrate that wild-type let-7 microRNA binds in vitro to RNA from the lin-41 3′UTR. This interaction is dependent on two conserved let-7 complementary sites (LCSs). A 27-nucleotide sequence between the LCSs is also necessary for down-regulation in vivo. LCS mutations compensatory to the lesion in let-7(n2853) can partially restore lin-41 3′UTR function in a let-7(n2853) background, providing the first experimental evidence for an animal miRNA binding directly to its validated target in vivo.

Original languageEnglish (US)
Pages (from-to)132-137
Number of pages6
JournalGenes and Development
Volume18
Issue number2
DOIs
StatePublished - Jan 15 2004

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Keywords

  • 3′UTR
  • Developmental timing
  • Heterochronic
  • MicroRNA
  • StRNA

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