@article{8b9c05d8e1164d58ac0e266e99c20a0a,
title = "The bright frontiers of microbial metabolic optogenetics",
abstract = "In recent years, light-responsive systems from the field of optogenetics have been applied to several areas of metabolic engineering with remarkable success. By taking advantage of light's high tunability, reversibility, and orthogonality to host endogenous processes, optogenetic systems have enabled unprecedented dynamical controls of microbial fermentations for chemical production, metabolic flux analysis, and population compositions in co-cultures. In this article, we share our opinions on the current state of this new field of metabolic optogenetics.We make the case that it will continue to impact metabolic engineering in increasingly new directions, with the potential to challenge existing paradigms for metabolic pathway and strain optimization as well as bioreactor operation.",
keywords = "Biosensors, Control, Cybergenetics, DBTL, Dynamic control of microbial fermentations: Design build test learn cycle, MMME, Metabolic engineering, Multivariate modular metabolic engineering, Optogenetics",
author = "Wegner, {Scott A.} and Barocio-Galindo, {Rachel M.} and Avalos, {Jos{\'e} L.}",
note = "Funding Information: Jose L. Avalos reports financial support was provided by US Department of Energy, as well as the National Science Foundation. Scott A. Wegner reports financial support was provided by National Institutes of Health. Jose L. Avalos has patents pending to Princeton University. Funding Information: We thank Saurabh Malani for assistance in preparing Fig. 3 , and members of the Avalos lab for their input in early preparations of this manuscript. The authors and their research are supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research (Award Numbers DE-SC0019363 and DE-SC0022155 ), the Air Force Office of Scientific Research ( FA9550 -20-1-0241 ), the NSF CAREER Award CBET-1751840 , as well as by the NIGMS of the National Institutes of Health under grant number T32GM007388 (to S.A.W.). The content of this article is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Publisher Copyright: {\textcopyright} 2022 Elsevier Ltd",
year = "2022",
month = dec,
doi = "10.1016/j.cbpa.2022.102207",
language = "English (US)",
volume = "71",
journal = "Current Opinion in Chemical Biology",
issn = "1367-5931",
publisher = "Elsevier Limited",
}