TY - JOUR
T1 - The alliance for cellular signaling plasmid collection
T2 - A flexible resource for protein localization studies and signalling pathway analysis
AU - Zavzavadjian, Joelle R.
AU - Couture, Sam
AU - Park, Wei Sun
AU - Whalen, James
AU - Lyon, Stephen
AU - Lee, Genie
AU - Fung, Eileen
AU - Mi, Qingli
AU - Liu, Jamie
AU - Wall, Estelle
AU - Santat, Leah
AU - Dhandapani, Kavitha
AU - Kivork, Christine
AU - Driver, Adrienne
AU - Zhu, Xiaocui
AU - Chang, Mi Sook
AU - Randhawa, Baljinder
AU - Gehrig, Elizabeth
AU - Bryan, Heather
AU - Verghese, Mary
AU - Maer, Andreia
AU - Saunders, Brian
AU - Ning, Yuhong
AU - Subramaniam, Shankar
AU - Meyer, Tobias
AU - Simon, Melvin I.
AU - O'Rourke, Nancy
AU - Chandy, Grischa
AU - Fraser, Ian D.C.
PY - 2007/3
Y1 - 2007/3
N2 - Cellular responses to inputs that vary both temporally and spatially are determined by complex relationships between the components of cell signaling networks. Analysis of these relationships requires access to a wide range of experimental reagents and techniques, including the ability to express the protein components of the model cells in a variety of contexts. As part of the Alliance for Cellular Signaling, we developed a robust method for cloning large numbers of signaling ORFs into Gateway® entry vectors, and we created a wide range of compatible expression platforms for proteomics applications. To date, we have generated over 3000 plasmids that are available to the scientific community via the American Type Culture Collection. We have established a website at www.signaling-gateway.org/data/plasmid/ that allows users to browse, search, and blast Alliance for Cellular Signaling plasmids. The collection primarily contains murine signaling ORFs with an emphasis on kinases and G protein signaling genes. Here we describe the cloning, databasing, and application of this proteomics resource for large scale subcellular localization screens in mammalian cell lines.
AB - Cellular responses to inputs that vary both temporally and spatially are determined by complex relationships between the components of cell signaling networks. Analysis of these relationships requires access to a wide range of experimental reagents and techniques, including the ability to express the protein components of the model cells in a variety of contexts. As part of the Alliance for Cellular Signaling, we developed a robust method for cloning large numbers of signaling ORFs into Gateway® entry vectors, and we created a wide range of compatible expression platforms for proteomics applications. To date, we have generated over 3000 plasmids that are available to the scientific community via the American Type Culture Collection. We have established a website at www.signaling-gateway.org/data/plasmid/ that allows users to browse, search, and blast Alliance for Cellular Signaling plasmids. The collection primarily contains murine signaling ORFs with an emphasis on kinases and G protein signaling genes. Here we describe the cloning, databasing, and application of this proteomics resource for large scale subcellular localization screens in mammalian cell lines.
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U2 - 10.1074/mcp.M600437-MCP200
DO - 10.1074/mcp.M600437-MCP200
M3 - Article
C2 - 17192258
AN - SCOPUS:34147186844
SN - 1535-9476
VL - 6
SP - 413
EP - 424
JO - Molecular and Cellular Proteomics
JF - Molecular and Cellular Proteomics
IS - 3
ER -