The adenovirus type 5 E1A transcriptional control region contains a duplicated enhancer element

Patrick Hearing, Thomas Shenk

Research output: Contribution to journalArticle

211 Scopus citations

Abstract

The adenovirus type 5 EIA transcriptional control region contains an element with enhancer properties located between -141 and -305 relative to the E1A cap site at +1. The enhancer element is located at or very close to a sequence required in cis for packaging of viral DNA. Deletion of the element reduces both the rate of transcription and steady-state levels of EIA mRNAs in virus-infected cells. Such deletions also cause a modest reduction in activity of the E1B transcription unit, which is located immediately downstream of the EIA unit. The enhancer element includes repeated core sequences. Function is retained when the element is moved to the 3′ side of the E1A gene in either possible orientation, and the E1A enhancer functions in cis to enhance transformation by the herpesvirus thymidine kinase gene in both mouse and human cells.

Original languageEnglish (US)
Pages (from-to)695-703
Number of pages9
JournalCell
Volume33
Issue number3
DOIs
StatePublished - Jul 1983
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'The adenovirus type 5 E1A transcriptional control region contains a duplicated enhancer element'. Together they form a unique fingerprint.

  • Cite this