The adenovirus tripartite leader sequence can alter nuclear and cytoplasmic metabolism of a non-adenovirus mRNA within infected cells

Mary A. Moore, Thomas Shenk

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

All mRNAs encoded by the adenovirus major late transcription unit share a common 5' noncoding region, 200 nucleotides in length, termed the tripartite leader sequence. To assess function of the tripartite leader, recombinant viruses were prepared which carried either a bona fide herpes simplex virus thymidine kinase gene or a modified thymidine kinase gene whose normal 5' noncoding domain was replaced with the adenovirus leader sequence. The tripartite leader simultaneously decreased the nuclear half-life and increased the cytoplasmic half-life of the thymidine kinase-specific mRNA. The tripartite leader stabilized the non-adenovirus inRNA only within the environment of an adenovirus-infected cell during the late phase of the infectious cycle.

Original languageEnglish (US)
Pages (from-to)2247-2262
Number of pages16
JournalNucleic acids research
Volume16
Issue number5
DOIs
StatePublished - Mar 11 1988

All Science Journal Classification (ASJC) codes

  • Genetics

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