The A2aR adenosine receptor controls cytokine production in iNKT cells

Michael Nowak, Lydia Lynch, Simon Yue, Akio Ohta, Michail Sitkovsky, Steven P. Balk, Mark A. Exley

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

The purine nucleoside adenosine is an important anti-inflammatory molecule, inhibiting a variety of immune cells by adenosine receptor-mediated mechanisms. Invariant NKT (iNKT) cells recognize glycolipids presented on CD1d molecules and produce vigorous amounts of cytokines upon activation, hence regulating immune reactions. The mechanisms polarizing their cytokine pattern are elusive. Previous studies demonstrated that adenosine can suppress IFN-γ production by iNKT cells.We describe the expression of all four known adenosine receptors A1R, A2aR, A2bR and A3R on mouse iNKT cells. We show that IL-4 production in primary mouse iNKT cells and a human iNKT line is effi-ciently inhibited by A2aR blockade with an inverse relation to IL-4. These data are supported by A2aR-deficient mice, which exhibit largely decreased levels of IL-4, IL-10 and TGF-β concomitantly with an increase of IFN-γ upon α-galactosylceramide administration in vivo. While A2aR inhibits other lymphocyte populations, A2aR is required for the secretion of IL-4 and IL-10 by iNKT cells. These data suggest adenosine:A2aR-mediated mechanisms can control the cytokine secretion pattern of iNKT cells.

Original languageEnglish (US)
Pages (from-to)682-687
Number of pages6
JournalEuropean Journal of Immunology
Volume40
Issue number3
DOIs
StatePublished - Mar 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Keywords

  • Cellular activation
  • Immune regulation
  • NKT cells

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