TY - JOUR
T1 - The 5S genes of drosophila melanogaster
AU - Artavanis-Tsakonas, Spyros
AU - Schedl, Paul
AU - Tschudi, Christian
AU - Pirrotta, Vincenzo
AU - Steward, Ruth
AU - Gehring, Walter J.
N1 - Funding Information:
This work was supported by grants from the Swiss National Science Foundation (to W.J.G. and V.P.). P.S. is a postdoctoral fellow of the Helen Hay Whitney Foundation, and S.A.-T. was supported by a long-term fellowship from EMBO. We thank Drs. David Hogness and G. Rubin for advice in the cloning techniques, Dr. S. lida for Pl DNA, and H. Heusser, G. Martin, M. Schedl, K. lneichen and E. Wenger for excellent technical assistance. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
PY - 1977/12
Y1 - 1977/12
N2 - We have cloned embryonic Drosophila DNA using the poly (dA-dT) connector method (Lobban and Kaiser, 1973) and the ampicillin-resistant plasmid pSF2124 (So, Gill and Falkow, 1975) as a cloning vehicle. Two clones, containing hybrid plasmids with sequences complementary to a 5S RNA probe isolated from Drosophila tissue culture cells, were identified by the Grunstein and Hogness (1975) colony hybridization procedure. One hybrid plasmid has a Drosophila insert which is comprised solely of tandem repeats of the 5S gene plus spacer sequences. The other plasmid contains an insert which has about 20 tandem 5S repeat units plus an additional 4 kilobases of adjacent sequences. The size of the 5S repeat unit was determined by gel electrophoresis and was found to be approximately 375 base pairs. We present a restriction map of both plasmids, and a detailed map of the 5S repeat unit. The 5S repeat unit shows slight length and sequence heterogeneity. We present evidence suggesting that the 5S genes in Drosophila melanogaster may be arranged in a single continuous cluster.
AB - We have cloned embryonic Drosophila DNA using the poly (dA-dT) connector method (Lobban and Kaiser, 1973) and the ampicillin-resistant plasmid pSF2124 (So, Gill and Falkow, 1975) as a cloning vehicle. Two clones, containing hybrid plasmids with sequences complementary to a 5S RNA probe isolated from Drosophila tissue culture cells, were identified by the Grunstein and Hogness (1975) colony hybridization procedure. One hybrid plasmid has a Drosophila insert which is comprised solely of tandem repeats of the 5S gene plus spacer sequences. The other plasmid contains an insert which has about 20 tandem 5S repeat units plus an additional 4 kilobases of adjacent sequences. The size of the 5S repeat unit was determined by gel electrophoresis and was found to be approximately 375 base pairs. We present a restriction map of both plasmids, and a detailed map of the 5S repeat unit. The 5S repeat unit shows slight length and sequence heterogeneity. We present evidence suggesting that the 5S genes in Drosophila melanogaster may be arranged in a single continuous cluster.
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U2 - 10.1016/0092-8674(77)90169-6
DO - 10.1016/0092-8674(77)90169-6
M3 - Article
C2 - 413625
AN - SCOPUS:0017751306
SN - 0092-8674
VL - 12
SP - 1057
EP - 1067
JO - Cell
JF - Cell
IS - 4
ER -