TY - JOUR
T1 - The 2022 Report on the Human Proteome from the HUPO Human Proteome Project
AU - Omenn, Gilbert S.
AU - Lane, Lydie
AU - Overall, Christopher M.
AU - Pineau, Charles
AU - Packer, Nicolle H.
AU - Cristea, Ileana M.
AU - Lindskog, Cecilia
AU - Weintraub, Susan T.
AU - Orchard, Sandra
AU - Roehrl, Michael H.A.
AU - Nice, Edouard
AU - Liu, Siqi
AU - Bandeira, Nuno
AU - Chen, Yu Ju
AU - Guo, Tiannan
AU - Aebersold, Ruedi
AU - Moritz, Robert L.
AU - Deutsch, Eric W.
N1 - Funding Information:
We appreciate the guidance from the HPP Executive Committee and the participation of all HPP investigators. We thank the UniProt groups at SIB, EBI, and PIR for providing high-quality annotations for the human proteins in UniProtKB/Swiss-Prot. The neXtProt server is hosted at SIB Swiss Institute of Bioinformatics in Switzerland, ProteomeXchange and PRIDE at the European Bioinformatics Institute in Cambridge, U.K., PeptideAtlas at the Institute for Systems Biology in Seattle, and MassIVE at the University of California San Diego. G.S.O. acknowledges support from National Institutes of Health Grants P30ES017885-01A1 and U24CA210967; E.W.D. and R.L.M. from National Institutes of Health Grants R01GM087221, R24GM127667, U19AG023122, S10OD026936, and from National Science Foundation Grant DBI-1933311; C.M.O. by Canadian Institutes of Health Research Foundation Grant 148408 and a Canada Research Chair in Protease Proteomics and Systems Biology; N.B. from NIH grant 1R01LM013115, and NSF grant ABI 1759980; M.S.B. by Australian Research Council (CE140100003); C.L. by the Knut and Alice Wallenberg Foundation for the Human Protein Atlas; M.H.R by National Institutes of Health Grants R21 CA263262, U01 CA253217, R21 CA251992, P30 CA008748 (MSKCC CCSG, Pathology Component), NIH-Leidos CPTAC contract 17 × 173, and Farmer Family Foundation; and I.M.C. from National Institutes of Health Grant R01GM114141 and Stand Up To Cancer Convergence 3.1416.
Publisher Copyright:
© 2022 American Chemical Society.
PY - 2022
Y1 - 2022
N2 - The 2022 Metrics of the Human Proteome from the HUPO Human Proteome Project (HPP) show that protein expression has now been credibly detected (neXtProt PE1 level) for 18407 (93.2%) of the 19750 predicted proteins coded in the human genome, a net gain of 50 since 2021 from data sets generated around the world and reanalyzed by the HPP. Conversely, the number of neXtProt PE2, PE3, and PE4 missing proteins has been reduced by 78 from 1421 to 1343. This represents continuing experimental progress on the human proteome parts list across all the chromosomes, as well as significant reclassifications. Meanwhile, applying proteomics in a vast array of biological and clinical studies continues to yield significant findings and growing integration with other omics platforms. We present highlights from the Chromosome-Centric HPP, Biology and Disease-driven HPP, and HPP Resource Pillars, compare features of mass spectrometry and Olink and Somalogic platforms, note the emergence of translation products from ribosome profiling of small open reading frames, and discuss the launch of the initial HPP Grand Challenge Project, "A Function for Each Protein".
AB - The 2022 Metrics of the Human Proteome from the HUPO Human Proteome Project (HPP) show that protein expression has now been credibly detected (neXtProt PE1 level) for 18407 (93.2%) of the 19750 predicted proteins coded in the human genome, a net gain of 50 since 2021 from data sets generated around the world and reanalyzed by the HPP. Conversely, the number of neXtProt PE2, PE3, and PE4 missing proteins has been reduced by 78 from 1421 to 1343. This represents continuing experimental progress on the human proteome parts list across all the chromosomes, as well as significant reclassifications. Meanwhile, applying proteomics in a vast array of biological and clinical studies continues to yield significant findings and growing integration with other omics platforms. We present highlights from the Chromosome-Centric HPP, Biology and Disease-driven HPP, and HPP Resource Pillars, compare features of mass spectrometry and Olink and Somalogic platforms, note the emergence of translation products from ribosome profiling of small open reading frames, and discuss the launch of the initial HPP Grand Challenge Project, "A Function for Each Protein".
KW - Biology and Disease-HPP (B/D-HPP)
KW - chromosome-centric HPP (C-HPP)
KW - Grand Challenge Project
KW - Human Protein Atlas
KW - Human Proteome Project (HPP)
KW - Mass Spectrometry Interactive Virtual Environment (MassIVE)
KW - missing proteins (MP)
KW - neXtProt protein existence (PE metrics)
KW - non-MS PE1 proteins
KW - PeptideAtlas
KW - Ribo-Seq
KW - small open reading frames (smORFs)
KW - uncharacterized protein existence 1 (uPE1)
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U2 - 10.1021/acs.jproteome.2c00498
DO - 10.1021/acs.jproteome.2c00498
M3 - Review article
C2 - 36318223
AN - SCOPUS:85141716437
SN - 1535-3893
JO - Journal of Proteome Research
JF - Journal of Proteome Research
ER -