TY - JOUR
T1 - TATA-binding protein-independent initiation
T2 - YY1, TFIIB, and RNA polymerase II direct basal transcription on supercoiled template DNA
AU - Usheva, Anny
AU - Shenk, Thomas
N1 - Funding Information:
Correspondence should be addressed to T. S. We dedicate this paper to the memory of Y. Aloni, mentor and friend. We thank A. Hirsch and B. Oronsky for help with experiments; A. Berk. Z. Burton, J. Flint, H. Houbavi, R. Pruzan, D. Reinberg, M. Sawadogo, and R. Weinmann for reagents; and A. Hirsch, N. Horlkoshi, and H. Zhu for helpful discussions. This work was supported by Public Health Sewtce grant CA36965 from the National Cancer Institute. T. S. is an American Cancer Society professor and an Investigator of the Howard Hughes Medical Institute.
PY - 1994/3/25
Y1 - 1994/3/25
N2 - YY1 is a zinc finger transcription factor whose DNA-binding motif exhibits the properties of an initiator element. Only three factors were required to direct specific basal transcription on a supercoiled template DNA carrying the YY1 initiator: YY1, general transcription factor IIB, and RNA polymerase II. This minimal in vitro reaction did not require the TATA-binding protein (TBP). We propose that, under appropriate conditions, YY1 can function like TBP, as a factor that binds to the core promoter and recruits the polymerase to the initiation complex.
AB - YY1 is a zinc finger transcription factor whose DNA-binding motif exhibits the properties of an initiator element. Only three factors were required to direct specific basal transcription on a supercoiled template DNA carrying the YY1 initiator: YY1, general transcription factor IIB, and RNA polymerase II. This minimal in vitro reaction did not require the TATA-binding protein (TBP). We propose that, under appropriate conditions, YY1 can function like TBP, as a factor that binds to the core promoter and recruits the polymerase to the initiation complex.
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U2 - 10.1016/0092-8674(94)90387-5
DO - 10.1016/0092-8674(94)90387-5
M3 - Article
C2 - 8137426
AN - SCOPUS:0028223852
VL - 76
SP - 1115
EP - 1121
JO - Cell
JF - Cell
SN - 0092-8674
IS - 6
ER -