Tandem repeated DNA in an intergenic region of Herpes simplex virus type 1 (Patton)

K. Umene; R. J. Watson; L. W. Enquist

doi:10.1016/0378-1119(84)90102-1

Tandem repeated DNA in an intergenic region of Herpes simplex virus type 1 (Patton)

K. Umene, R. J. Watson, L. W. Enquist

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25 Scopus citations

Abstract

When the entire U_s region of HSV-1 (Patton) was cloned as an EcoRI fragment in bacteriophage λgtWES, the BamHI B6B5 fragment was observed to vary in size among independent isolates [Umene and Enquist, Gene 13 (1981) 251-268]. This fragment polymorphism also occurred in DNA of HSV-1 single plaque isolates. We report here that this heterogeneity is due to variation in copy number of a 15-bp tandem repeat of sequence 5'-CCACTCCCCACCCAC-3', which apparently lies in an intergenic region of the HSV-1 DNA.

Original language	English (US)
Pages (from-to)	33-39
Number of pages	7
Journal	Gene
Volume	30
Issue number	1-3
DOIs	https://doi.org/10.1016/0378-1119(84)90102-1
State	Published - Oct 1984

All Science Journal Classification (ASJC) codes

Genetics

Keywords

Recombinant DNA
Southern transfer hybridization
fragment polymorphism

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10.1016/0378-1119(84)90102-1

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title = "Tandem repeated DNA in an intergenic region of Herpes simplex virus type 1 (Patton)",

abstract = "When the entire Us region of HSV-1 (Patton) was cloned as an EcoRI fragment in bacteriophage λgtWES, the BamHI B6B5 fragment was observed to vary in size among independent isolates [Umene and Enquist, Gene 13 (1981) 251-268]. This fragment polymorphism also occurred in DNA of HSV-1 single plaque isolates. We report here that this heterogeneity is due to variation in copy number of a 15-bp tandem repeat of sequence 5'-CCACTCCCCACCCAC-3', which apparently lies in an intergenic region of the HSV-1 DNA.",

keywords = "Recombinant DNA, Southern transfer hybridization, fragment polymorphism",

author = "K. Umene and Watson, \{R. J.\} and Enquist, \{L. W.\}",

year = "1984",

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doi = "10.1016/0378-1119(84)90102-1",

language = "English (US)",

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pages = "33--39",

journal = "Gene",

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T1 - Tandem repeated DNA in an intergenic region of Herpes simplex virus type 1 (Patton)

AU - Umene, K.

AU - Watson, R. J.

AU - Enquist, L. W.

PY - 1984/10

Y1 - 1984/10

N2 - When the entire Us region of HSV-1 (Patton) was cloned as an EcoRI fragment in bacteriophage λgtWES, the BamHI B6B5 fragment was observed to vary in size among independent isolates [Umene and Enquist, Gene 13 (1981) 251-268]. This fragment polymorphism also occurred in DNA of HSV-1 single plaque isolates. We report here that this heterogeneity is due to variation in copy number of a 15-bp tandem repeat of sequence 5'-CCACTCCCCACCCAC-3', which apparently lies in an intergenic region of the HSV-1 DNA.

AB - When the entire Us region of HSV-1 (Patton) was cloned as an EcoRI fragment in bacteriophage λgtWES, the BamHI B6B5 fragment was observed to vary in size among independent isolates [Umene and Enquist, Gene 13 (1981) 251-268]. This fragment polymorphism also occurred in DNA of HSV-1 single plaque isolates. We report here that this heterogeneity is due to variation in copy number of a 15-bp tandem repeat of sequence 5'-CCACTCCCCACCCAC-3', which apparently lies in an intergenic region of the HSV-1 DNA.

KW - Recombinant DNA

KW - Southern transfer hybridization

KW - fragment polymorphism

UR - https://www.scopus.com/pages/publications/0021738741

UR - https://www.scopus.com/inward/citedby.url?scp=0021738741&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(84)90102-1

DO - 10.1016/0378-1119(84)90102-1

M3 - Article

C2 - 6096222

AN - SCOPUS:0021738741

SN - 0378-1119

VL - 30

SP - 33

EP - 39

JO - Gene

JF - Gene

IS - 1-3

ER -

Tandem repeated DNA in an intergenic region of Herpes simplex virus type 1 (Patton)

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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