Tandem repeated DNA in an intergenic region of Herpes simplex virus type 1 (Patton)

K. Umene, R. J. Watson, L. W. Enquist

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

When the entire Us region of HSV-1 (Patton) was cloned as an EcoRI fragment in bacteriophage λgtWES, the BamHI B6B5 fragment was observed to vary in size among independent isolates [Umene and Enquist, Gene 13 (1981) 251-268]. This fragment polymorphism also occurred in DNA of HSV-1 single plaque isolates. We report here that this heterogeneity is due to variation in copy number of a 15-bp tandem repeat of sequence 5'-CCACTCCCCACCCAC-3', which apparently lies in an intergenic region of the HSV-1 DNA.

Original languageEnglish (US)
Pages (from-to)33-39
Number of pages7
JournalGene
Volume30
Issue number1-3
DOIs
StatePublished - Oct 1984
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

Keywords

  • Recombinant DNA
  • Southern transfer hybridization
  • fragment polymorphism

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