@article{9bb9f6d63d754327b6eda17675e610fb,
title = "Systematic characterization of gene function in the photosynthetic alga Chlamydomonas reinhardtii",
abstract = "Most genes in photosynthetic organisms remain functionally uncharacterized. Here, using a barcoded mutant library of the model eukaryotic alga Chlamydomonas reinhardtii, we determined the phenotypes of more than 58,000 mutants under more than 121 different environmental growth conditions and chemical treatments. A total of 59% of genes are represented by at least one mutant that showed a phenotype, providing clues to the functions of thousands of genes. Mutant phenotypic profiles place uncharacterized genes into functional pathways such as DNA repair, photosynthesis, the CO2-concentrating mechanism and ciliogenesis. We illustrate the value of this resource by validating phenotypes and gene functions, including three new components of an actin cytoskeleton defense pathway. The data also inform phenotype discovery in land plants; mutants in Arabidopsis thaliana genes exhibit phenotypes similar to those we observed in their Chlamydomonas homologs. We anticipate that this resource will guide the functional characterization of genes across the tree of life.",
author = "Friedrich Fauser and Josep Vilarrasa-Blasi and Masayuki Onishi and Silvia Ramundo and Weronika Patena and Matthew Millican and Jacqueline Osaki and Charlotte Philp and Matthew Nemeth and Salom{\'e}, {Patrice A.} and Xiaobo Li and Setsuko Wakao and Kim, {Rick G.} and Yuval Kaye and Grossman, {Arthur R.} and Niyogi, {Krishna K.} and Merchant, {Sabeeha S.} and Cutler, {Sean R.} and Peter Walter and Dinneny, {Jos{\'e} R.} and Jonikas, {Martin C.} and Jinkerson, {Robert E.}",
note = "Funding Information: We thank M. Cahn for developing and improving the CLiP website; X. Ji at the Stanford Functional Genomics Facility and Z. Weng at the Stanford Center for Genomics and Personalized Medicine for deep-sequencing services; K. Barton, W. Briggs and Z.-Y. Wang for providing lab space; T. Raab for providing lab equipment; J. Robertson and N. Ivanova for helping with library maintenance; M. Sison-Mangus for advice on Daphnia experiments; T. Kato-Minoura and R. Kamiya for the anti-NAP1 antibody; J. R. Pringle for supporting M.O.; J. Whitney for assistance in figure preparation; and members of the Dinneny, Jonikas and Jinkerson Labs and T. Xiang for constructive suggestions on the manuscript. This project was supported by grants from the National Institutes of Health (DP2-GM-119137), the National Science Foundation (MCB-1914989 and MCB-1146621) and the Simons Foundation and Howard Hughes Medical Institute (55108535) awarded to M.C.J.; a German Academic Exchange Service (DAAD) research fellowship awarded to F.F.; Simons Foundation fellowships of the Life Sciences Research Foundation awarded to R.E.J. and J.V.-B.; an EMBO long-term fellowship (ALTF 1450-2014 and ALTF 563-2013) awarded to J.V.-B. and S.R.; an National Science Foundation MCB Grant (1818383) awarded to M.O.; a Swiss National Science Foundation Advanced PostDoc Mobility Fellowship (P2GEP3_148531) awarded to S.R.; and a Simons Foundation and Howard Hughes Medical Institute (55108515) Faculty Scholars grant awarded to J.R.D. S.W. was supported by the U.S. Department of Energy, Office of Science, Basic Energy Sciences, Chemical Sciences, Geosciences, and Biosciences Division under field work proposal 449B. K.K.N. is an investigator at the Howard Hughes Medical Institute. Work in the Merchant laboratory is supported by a cooperative agreement with the US Department of Energy Office of Science, Office of Biological and Environmental Research program under award DE-FC02-02ER63421. Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
month = may,
doi = "10.1038/s41588-022-01052-9",
language = "English (US)",
volume = "54",
pages = "705--714",
journal = "Nature Genetics",
issn = "1061-4036",
publisher = "Nature Publishing Group",
number = "5",
}