TY - JOUR
T1 - Surface-attached molecules control Staphylococcus aureus quorum sensing and biofilm development
AU - Kim, Minyoung Kevin
AU - Zhao, Aishan
AU - Wang, Ashley
AU - Brown, Zachary Z.
AU - Muir, Thomas William
AU - Stone, Howard A.
AU - Bassler, Bonnie Lynn
PY - 2017/5/22
Y1 - 2017/5/22
N2 - Bacteria use a process called quorum sensing to communicate and orchestrate collective behaviours, including virulence factor secretion and biofilm formation. Quorum sensing relies on the production, release, accumulation and population-wide detection of signal molecules called autoinducers. Here, we develop concepts to coat surfaces with quorum-sensing-manipulation molecules as a method to control collective behaviours. We probe this strategy using Staphylococcus aureus. Pro- and anti-quorum-sensing molecules can be covalently attached to surfaces using click chemistry, where they retain their abilities to influence bacterial behaviours. We investigate key features of the compounds, linkers and surfaces necessary to appropriately position molecules to interact with cognate receptors and the ability of modified surfaces to resist long-term storage, repeated infections, host plasma components and flow-generated stresses. Our studies highlight how this surface approach can be used to make colonization-resistant materials against S. aureus and other pathogens and how the approach can be adapted to promote beneficial behaviours of bacteria on surfaces.
AB - Bacteria use a process called quorum sensing to communicate and orchestrate collective behaviours, including virulence factor secretion and biofilm formation. Quorum sensing relies on the production, release, accumulation and population-wide detection of signal molecules called autoinducers. Here, we develop concepts to coat surfaces with quorum-sensing-manipulation molecules as a method to control collective behaviours. We probe this strategy using Staphylococcus aureus. Pro- and anti-quorum-sensing molecules can be covalently attached to surfaces using click chemistry, where they retain their abilities to influence bacterial behaviours. We investigate key features of the compounds, linkers and surfaces necessary to appropriately position molecules to interact with cognate receptors and the ability of modified surfaces to resist long-term storage, repeated infections, host plasma components and flow-generated stresses. Our studies highlight how this surface approach can be used to make colonization-resistant materials against S. aureus and other pathogens and how the approach can be adapted to promote beneficial behaviours of bacteria on surfaces.
UR - http://www.scopus.com/inward/record.url?scp=85019660155&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85019660155&partnerID=8YFLogxK
U2 - 10.1038/nmicrobiol.2017.80
DO - 10.1038/nmicrobiol.2017.80
M3 - Article
C2 - 28530651
AN - SCOPUS:85019660155
SN - 2058-5276
VL - 2
JO - Nature Microbiology
JF - Nature Microbiology
ER -