[3H]3,4-Methylenedioxymethamphetamine (MDMA) interactions with brain membranes and glass fiber filter paper

Samuel S. Wang; George A. Ricaurte; Stephen J. Peroutka

doi:10.1016/0014-2999(87)90485-7

[³H]3,4-Methylenedioxymethamphetamine (MDMA) interactions with brain membranes and glass fiber filter paper

Samuel S. Wang, George A. Ricaurte, Stephen J. Peroutka

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The binding of [³H]3,4-methylenedioxymethamphetamine ([³H]MDMA) to both rat brain membrane preparations and glass fiber filter papers was analyzed in the present study. Saturation studies indicate that [³H]MDMA binding is saturable and monophasic in both the presence and absence of rat brain membranes. This apparent 'specific' binding of [³H]MDMA in both the presence and absence of brain homogenates was totally eliminated by pretreating glass fiber filter papers with polyethylenimine. Drug competition studies demonstrated that [³H]MDMA binding displays a distinct 'pharmacological' profile in the absence of brain tissue. These data indicate that apparent [³H]MDMA binding to rat brain homogenates results from artifactual of [³H]MDMA to glass fiber filter paper. In addition, uptake of [³H]MDMA into rat brain synaptosomes could not be detected. We conclude the [³H]MDMA has limited usefulness in radioligand binding studies.

Original language	English (US)
Pages (from-to)	439-443
Number of pages	5
Journal	European Journal of Pharmacology
Volume	138
Issue number	3
DOIs	https://doi.org/10.1016/0014-2999(87)90485-7
State	Published - Jun 26 1987
Externally published	Yes

All Science Journal Classification (ASJC) codes

Pharmacology

Keywords

(Artifactual binding)
3,4-Methylenedioxymethamphetamine (MDMA)
Polyethylenimine
Radioligand binding studies

Access to Document

10.1016/0014-2999(87)90485-7

Cite this

@article{8986ef7a5f734d249910b648d377e022,

title = "[3H]3,4-Methylenedioxymethamphetamine (MDMA) interactions with brain membranes and glass fiber filter paper",

abstract = "The binding of [3H]3,4-methylenedioxymethamphetamine ([3H]MDMA) to both rat brain membrane preparations and glass fiber filter papers was analyzed in the present study. Saturation studies indicate that [3H]MDMA binding is saturable and monophasic in both the presence and absence of rat brain membranes. This apparent 'specific' binding of [3H]MDMA in both the presence and absence of brain homogenates was totally eliminated by pretreating glass fiber filter papers with polyethylenimine. Drug competition studies demonstrated that [3H]MDMA binding displays a distinct 'pharmacological' profile in the absence of brain tissue. These data indicate that apparent [3H]MDMA binding to rat brain homogenates results from artifactual of [3H]MDMA to glass fiber filter paper. In addition, uptake of [3H]MDMA into rat brain synaptosomes could not be detected. We conclude the [3H]MDMA has limited usefulness in radioligand binding studies.",

keywords = "(Artifactual binding), 3,4-Methylenedioxymethamphetamine (MDMA), Polyethylenimine, Radioligand binding studies",

author = "Wang, {Samuel S.} and Ricaurte, {George A.} and Peroutka, {Stephen J.}",

note = "Funding Information: We thank Faith Smith for assistance in manuscript preparation. This work was supported in part by the John A. and George U Hartford Foundation, the McKnight Foundation and NIH Grants NS 12151-12 and NS 23560-01.",

year = "1987",

month = jun,

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doi = "10.1016/0014-2999(87)90485-7",

language = "English (US)",

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AU - Wang, Samuel S.

AU - Ricaurte, George A.

AU - Peroutka, Stephen J.

N1 - Funding Information: We thank Faith Smith for assistance in manuscript preparation. This work was supported in part by the John A. and George U Hartford Foundation, the McKnight Foundation and NIH Grants NS 12151-12 and NS 23560-01.

PY - 1987/6/26

Y1 - 1987/6/26

N2 - The binding of [3H]3,4-methylenedioxymethamphetamine ([3H]MDMA) to both rat brain membrane preparations and glass fiber filter papers was analyzed in the present study. Saturation studies indicate that [3H]MDMA binding is saturable and monophasic in both the presence and absence of rat brain membranes. This apparent 'specific' binding of [3H]MDMA in both the presence and absence of brain homogenates was totally eliminated by pretreating glass fiber filter papers with polyethylenimine. Drug competition studies demonstrated that [3H]MDMA binding displays a distinct 'pharmacological' profile in the absence of brain tissue. These data indicate that apparent [3H]MDMA binding to rat brain homogenates results from artifactual of [3H]MDMA to glass fiber filter paper. In addition, uptake of [3H]MDMA into rat brain synaptosomes could not be detected. We conclude the [3H]MDMA has limited usefulness in radioligand binding studies.

AB - The binding of [3H]3,4-methylenedioxymethamphetamine ([3H]MDMA) to both rat brain membrane preparations and glass fiber filter papers was analyzed in the present study. Saturation studies indicate that [3H]MDMA binding is saturable and monophasic in both the presence and absence of rat brain membranes. This apparent 'specific' binding of [3H]MDMA in both the presence and absence of brain homogenates was totally eliminated by pretreating glass fiber filter papers with polyethylenimine. Drug competition studies demonstrated that [3H]MDMA binding displays a distinct 'pharmacological' profile in the absence of brain tissue. These data indicate that apparent [3H]MDMA binding to rat brain homogenates results from artifactual of [3H]MDMA to glass fiber filter paper. In addition, uptake of [3H]MDMA into rat brain synaptosomes could not be detected. We conclude the [3H]MDMA has limited usefulness in radioligand binding studies.

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