Abstract
We have used [3H]2-deoxyglucose autoradiography to correlate the labeling of individual neurons with electrical activity within the central nervous system of a terrestrial mollusc, Limax maximus. In an electrically quiescent control preparation where a single neuron is impaled with a glass microelectrode but not stimulated, several somata are uniformly labeled at 3-5 times background. In preparations where a single cell is impaled and stimulated, one or more somata are heavily labeled with [3H]2-deoxyglucose at 10-50 times tissue background. This technique may be useful for surveying metabolically active neurons during spontaneous and driven electrical activity.
Original language | English (US) |
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Pages (from-to) | 416-420 |
Number of pages | 5 |
Journal | Brain Research |
Volume | 208 |
Issue number | 2 |
DOIs | |
State | Published - Mar 16 1981 |
All Science Journal Classification (ASJC) codes
- Clinical Neurology
- Molecular Biology
- General Neuroscience
- Developmental Biology
Keywords
- 2-deoxyglucose
- Limax maximus
- autoradiography
- cell labeling
- functional morphology
- glucose uptake
- mollusc
- radioautography