[3H]2-Deoxyglucose autoradiography in a molluscan nervous system

Stephen C. Reingold, Terence J. Sejnowski, Alan Gelperin, Darcy B. Kelley

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

We have used [3H]2-deoxyglucose autoradiography to correlate the labeling of individual neurons with electrical activity within the central nervous system of a terrestrial mollusc, Limax maximus. In an electrically quiescent control preparation where a single neuron is impaled with a glass microelectrode but not stimulated, several somata are uniformly labeled at 3-5 times background. In preparations where a single cell is impaled and stimulated, one or more somata are heavily labeled with [3H]2-deoxyglucose at 10-50 times tissue background. This technique may be useful for surveying metabolically active neurons during spontaneous and driven electrical activity.

Original languageEnglish (US)
Pages (from-to)416-420
Number of pages5
JournalBrain Research
Volume208
Issue number2
DOIs
StatePublished - Mar 16 1981

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Keywords

  • 2-deoxyglucose
  • Limax maximus
  • autoradiography
  • cell labeling
  • functional morphology
  • glucose uptake
  • mollusc
  • radioautography

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