@article{c65de8b4a5dc4eedbde732310faad0d6,
title = "Subunits of the pbap chromatin remodeler are capable of mediating enhancer-driven transcription in drosophila",
abstract = "The chromatin remodeler SWI/SNF is an important participant in gene activation, functioning predominantly by opening the chromatin structure on promoters and enhancers. Here, we describe its novel mode of action in which SWI/SNF factors mediate the targeted action of an enhancer. We studied the functions of two signature subunits of PBAP subfamily, BAP170 and SAYP, in Drosophila. These subunits were stably tethered to a transgene reporter carrying the hsp70 core promoter. The tethered subunits mediate transcription of the reporter in a pattern that is generated by enhancers close to the insertion site in multiple loci throughout the genome. Both tethered SAYP and BAP170 recruit the whole PBAP complex to the reporter promoter. However, we found that BAP170-dependent transcription is more resistant to the depletion of other PBAP subunits, suggesting that BAP170 may play a more critical role in establishing enhancer-dependent transcription.",
keywords = "Chromatin remodeling, Enhancer, PBAP, Promoter, SWI/SNF",
author = "Shidlovskii, {Yulii V.} and Bylino, {Oleg V.} and Shaposhnikov, {Alexander V.} and Kachaev, {Zaur M.} and Lebedeva, {Lyubov A.} and Kolesnik, {Valeria V.} and Diego Amendola and {De Simone}, Giovanna and Nadia Formicola and Paul Schedl and Digilio, {Filomena Anna} and Ennio Giordano",
note = "Funding Information: Funding: This study was supported by the Russian Foundation for Basic Research (project 19-34-51003; chapters 2.5, 2.6), the Russian Science Foundation (project 20-14-00201; chapters 2.1, 2.4), and Italian POR “BioIndustrial Processess” (BIP). Funding Information: This study was supported by the Russian Foundation for Basic Research (project 19-34-51003; chapters 2.5, 2.6), the Russian Science Foundation (project 20-14-00201; chapters 2.1, 2.4), and Italian POR “BioIndustrial Processess” (BIP). We thank Sergey Ulianov (IGB RAS) for assistance with the 3C experiment. We thank Daniela Cavaliere and Orsolina Petillo for their technical support and Mariarosaria Aletta (CNR) for bibliographic support. We are grateful to the Bloomington stock Center for Drosophila strains. This study was performed using DM6 Leica microscopes at IBBR and the infrastructure of the Center for Collective Use “Biology of the Living Cell and Drug Biomedical Nanotransporters” of the Institute of Gene Biology RAS. We also thank the Center for Precision Genome Editing and Genetic Technologies for Biomedicine (Institute of Gene Biology RAS) for the equipment and ge-nome-editing technology. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2021",
month = mar,
day = "2",
doi = "10.3390/ijms22062856",
language = "English (US)",
volume = "22",
pages = "1--22",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "6",
}