Subunits of the pbap chromatin remodeler are capable of mediating enhancer-driven transcription in drosophila

Yulii V. Shidlovskii; Oleg V. Bylino; Alexander V. Shaposhnikov; Zaur M. Kachaev; Lyubov A. Lebedeva; Valeria V. Kolesnik; Diego Amendola; Giovanna De Simone; Nadia Formicola; Paul Schedl; Filomena Anna Digilio; Ennio Giordano

doi:10.3390/ijms22062856

Subunits of the pbap chromatin remodeler are capable of mediating enhancer-driven transcription in drosophila

Yulii V. Shidlovskii, Oleg V. Bylino, Alexander V. Shaposhnikov, Zaur M. Kachaev, Lyubov A. Lebedeva, Valeria V. Kolesnik, Diego Amendola, Giovanna De Simone, Nadia Formicola, Paul Schedl, Filomena Anna Digilio, Ennio Giordano

Molecular Biology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The chromatin remodeler SWI/SNF is an important participant in gene activation, functioning predominantly by opening the chromatin structure on promoters and enhancers. Here, we describe its novel mode of action in which SWI/SNF factors mediate the targeted action of an enhancer. We studied the functions of two signature subunits of PBAP subfamily, BAP170 and SAYP, in Drosophila. These subunits were stably tethered to a transgene reporter carrying the hsp70 core promoter. The tethered subunits mediate transcription of the reporter in a pattern that is generated by enhancers close to the insertion site in multiple loci throughout the genome. Both tethered SAYP and BAP170 recruit the whole PBAP complex to the reporter promoter. However, we found that BAP170-dependent transcription is more resistant to the depletion of other PBAP subunits, suggesting that BAP170 may play a more critical role in establishing enhancer-dependent transcription.

Original language	English (US)
Article number	2856
Pages (from-to)	1-22
Number of pages	22
Journal	International journal of molecular sciences
Volume	22
Issue number	6
DOIs	https://doi.org/10.3390/ijms22062856
State	Published - Mar 2 2021

All Science Journal Classification (ASJC) codes

Molecular Biology
Spectroscopy
Catalysis
Inorganic Chemistry
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry

Keywords

Chromatin remodeling
Enhancer
PBAP
Promoter
SWI/SNF

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10.3390/ijms22062856

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Shidlovskii, Y. V., Bylino, O. V., Shaposhnikov, A. V., Kachaev, Z. M., Lebedeva, L. A., Kolesnik, V. V., Amendola, D., De Simone, G., Formicola, N., Schedl, P., Digilio, F. A., & Giordano, E. (2021). Subunits of the pbap chromatin remodeler are capable of mediating enhancer-driven transcription in drosophila. International journal of molecular sciences, 22(6), 1-22. Article 2856. https://doi.org/10.3390/ijms22062856

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title = "Subunits of the pbap chromatin remodeler are capable of mediating enhancer-driven transcription in drosophila",

abstract = "The chromatin remodeler SWI/SNF is an important participant in gene activation, functioning predominantly by opening the chromatin structure on promoters and enhancers. Here, we describe its novel mode of action in which SWI/SNF factors mediate the targeted action of an enhancer. We studied the functions of two signature subunits of PBAP subfamily, BAP170 and SAYP, in Drosophila. These subunits were stably tethered to a transgene reporter carrying the hsp70 core promoter. The tethered subunits mediate transcription of the reporter in a pattern that is generated by enhancers close to the insertion site in multiple loci throughout the genome. Both tethered SAYP and BAP170 recruit the whole PBAP complex to the reporter promoter. However, we found that BAP170-dependent transcription is more resistant to the depletion of other PBAP subunits, suggesting that BAP170 may play a more critical role in establishing enhancer-dependent transcription.",

keywords = "Chromatin remodeling, Enhancer, PBAP, Promoter, SWI/SNF",

author = "Shidlovskii, {Yulii V.} and Bylino, {Oleg V.} and Shaposhnikov, {Alexander V.} and Kachaev, {Zaur M.} and Lebedeva, {Lyubov A.} and Kolesnik, {Valeria V.} and Diego Amendola and {De Simone}, Giovanna and Nadia Formicola and Paul Schedl and Digilio, {Filomena Anna} and Ennio Giordano",

note = "Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

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day = "2",

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Shidlovskii, YV, Bylino, OV, Shaposhnikov, AV, Kachaev, ZM, Lebedeva, LA, Kolesnik, VV, Amendola, D, De Simone, G, Formicola, N, Schedl, P, Digilio, FA & Giordano, E 2021, 'Subunits of the pbap chromatin remodeler are capable of mediating enhancer-driven transcription in drosophila', International journal of molecular sciences, vol. 22, no. 6, 2856, pp. 1-22. https://doi.org/10.3390/ijms22062856

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AU - Shidlovskii, Yulii V.

AU - Bylino, Oleg V.

AU - Shaposhnikov, Alexander V.

AU - Kachaev, Zaur M.

AU - Lebedeva, Lyubov A.

AU - Kolesnik, Valeria V.

AU - Amendola, Diego

AU - De Simone, Giovanna

AU - Formicola, Nadia

AU - Schedl, Paul

AU - Digilio, Filomena Anna

AU - Giordano, Ennio

PY - 2021/3/2

Y1 - 2021/3/2

N2 - The chromatin remodeler SWI/SNF is an important participant in gene activation, functioning predominantly by opening the chromatin structure on promoters and enhancers. Here, we describe its novel mode of action in which SWI/SNF factors mediate the targeted action of an enhancer. We studied the functions of two signature subunits of PBAP subfamily, BAP170 and SAYP, in Drosophila. These subunits were stably tethered to a transgene reporter carrying the hsp70 core promoter. The tethered subunits mediate transcription of the reporter in a pattern that is generated by enhancers close to the insertion site in multiple loci throughout the genome. Both tethered SAYP and BAP170 recruit the whole PBAP complex to the reporter promoter. However, we found that BAP170-dependent transcription is more resistant to the depletion of other PBAP subunits, suggesting that BAP170 may play a more critical role in establishing enhancer-dependent transcription.

AB - The chromatin remodeler SWI/SNF is an important participant in gene activation, functioning predominantly by opening the chromatin structure on promoters and enhancers. Here, we describe its novel mode of action in which SWI/SNF factors mediate the targeted action of an enhancer. We studied the functions of two signature subunits of PBAP subfamily, BAP170 and SAYP, in Drosophila. These subunits were stably tethered to a transgene reporter carrying the hsp70 core promoter. The tethered subunits mediate transcription of the reporter in a pattern that is generated by enhancers close to the insertion site in multiple loci throughout the genome. Both tethered SAYP and BAP170 recruit the whole PBAP complex to the reporter promoter. However, we found that BAP170-dependent transcription is more resistant to the depletion of other PBAP subunits, suggesting that BAP170 may play a more critical role in establishing enhancer-dependent transcription.

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KW - Enhancer

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KW - Promoter

KW - SWI/SNF

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Subunits of the pbap chromatin remodeler are capable of mediating enhancer-driven transcription in drosophila

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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