TY - JOUR
T1 - Subcellular localization of type IV pili regulates bacterial multicellular development
AU - Ellison, Courtney K.
AU - Fei, Chenyi
AU - Dalia, Triana N.
AU - Wingreen, Ned S.
AU - Dalia, Ankur B.
AU - Shaevitz, Joshua W.
AU - Gitai, Zemer
N1 - Funding Information:
We would like to thank the Proteomics and Mass Spectrometry Core Facility at Princeton University and S. Kyin for help with mass spectrometry. We would also like to thank the Confocal Microscopy Facility at Princeton University and G. Laevsky for help with confocal imaging. C.K.E. is a Damon Runyon Fellow supported by the Damon Runyon Cancer Research Foundation (DRG-2385-20). This work was supported in part by the National Science Foundation, through the Center for the Physics of Biological Function (PHY-1734030). This work was supported by National Institutes of Health grants R35GM128674 awarded to A.B.D. and R01 GM082938 awarded to N.S.W., and by National Institutes of Health Pioneer Award 1DP1AI124669-01 awarded to Z.G.
Funding Information:
We would like to thank the Proteomics and Mass Spectrometry Core Facility at Princeton University and S. Kyin for help with mass spectrometry. We would also like to thank the Confocal Microscopy Facility at Princeton University and G. Laevsky for help with confocal imaging. C.K.E. is a Damon Runyon Fellow supported by the Damon Runyon Cancer Research Foundation (DRG-2385-20). This work was supported in part by the National Science Foundation, through the Center for the Physics of Biological Function (PHY-1734030). This work was supported by National Institutes of Health grants R35GM128674 awarded to A.B.D. and R01 GM082938 awarded to N.S.W., and by National Institutes of Health Pioneer Award 1DP1AI124669-01 awarded to Z.G.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - In mammals, subcellular protein localization of factors like planar cell polarity proteins is a key driver of the multicellular organization of tissues. Bacteria also form organized multicellular communities, but these patterns are largely thought to emerge from regulation of whole-cell processes like growth, motility, cell shape, and differentiation. Here we show that a unique intracellular patterning of appendages known as type IV pili (T4P) can drive multicellular development of complex bacterial communities. Specifically, dynamic T4P appendages localize in a line along the long axis of the cell in the bacterium Acinetobacter baylyi. This long-axis localization is regulated by a functionally divergent chemosensory Pil-Chp system, and an atypical T4P protein homologue (FimV) bridges Pil-Chp signaling and T4P positioning. We further demonstrate through modeling and empirical approaches that subcellular T4P localization controls how individual cells interact with one another, independently of T4P dynamics, with different patterns of localization giving rise to distinct multicellular architectures. Our results reveal how subcellular patterning of single cells regulates the development of multicellular bacterial communities.
AB - In mammals, subcellular protein localization of factors like planar cell polarity proteins is a key driver of the multicellular organization of tissues. Bacteria also form organized multicellular communities, but these patterns are largely thought to emerge from regulation of whole-cell processes like growth, motility, cell shape, and differentiation. Here we show that a unique intracellular patterning of appendages known as type IV pili (T4P) can drive multicellular development of complex bacterial communities. Specifically, dynamic T4P appendages localize in a line along the long axis of the cell in the bacterium Acinetobacter baylyi. This long-axis localization is regulated by a functionally divergent chemosensory Pil-Chp system, and an atypical T4P protein homologue (FimV) bridges Pil-Chp signaling and T4P positioning. We further demonstrate through modeling and empirical approaches that subcellular T4P localization controls how individual cells interact with one another, independently of T4P dynamics, with different patterns of localization giving rise to distinct multicellular architectures. Our results reveal how subcellular patterning of single cells regulates the development of multicellular bacterial communities.
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U2 - 10.1038/s41467-022-33564-7
DO - 10.1038/s41467-022-33564-7
M3 - Article
C2 - 36284096
AN - SCOPUS:85140595982
SN - 2041-1723
VL - 13
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 6334
ER -