Structure of the motor subunit of type I restriction-modification complex EcoR124I

Mikalai Lapkouski, Santosh Panjikar, Pavel Janscak, Ivana Kuta Smatanova, Jannette Carey, Rüdiger Ettrich, Eva Csefalvay

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33 Scopus citations

Abstract

Type I restriction-modification enzymes act as conventional adenine methylases on hemimethylated DNAs, but unmethylated recognition targets induce them to translocate thousands of base pairs before cleaving distant sites nonspecifically. The first crystal structure of a type I motor subunit responsible for translocation and cleavage suggests how the pentameric translocating complex is assembled and provides a structural framework for translocation of duplex DNA by RecA-like ATPase motors.

Original languageEnglish (US)
Pages (from-to)94-95
Number of pages2
JournalNature Structural and Molecular Biology
Volume16
Issue number1
DOIs
StatePublished - Jan 1 2009

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

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    Lapkouski, M., Panjikar, S., Janscak, P., Smatanova, I. K., Carey, J., Ettrich, R., & Csefalvay, E. (2009). Structure of the motor subunit of type I restriction-modification complex EcoR124I. Nature Structural and Molecular Biology, 16(1), 94-95. https://doi.org/10.1038/nsmb.1523