Structure of the 70S ribosome complexed with mRNA and tRNA

Maria Selmer; Christine M. Dunham; Frank V. Murphy IV; Albert Weixlbaumer; Sabine Petry; Ann C. Kelley; John R. Weir; V. Ramakrishnan

doi:10.1126/science.1131127

Structure of the 70S ribosome complexed with mRNA and tRNA

Maria Selmer, Christine M. Dunham, Frank V. Murphy IV, Albert Weixlbaumer, Sabine Petry, Ann C. Kelley, John R. Weir, V. Ramakrishnan

Research output: Contribution to journal › Article › peer-review

1117 Scopus citations

Abstract

The crystal structure of the bacterial 70S ribosome refined to 2.8 angstrom resolution reveals atomic details of its interactions with messenger RNA (mRNA) and transfer RNA (tRNA). A metal ion stabilizes a kink in the mRNA that demarcates the boundary between A and P sites, which is potentially important to prevent slippage of mRNA. Metal ions also stabilize the intersubunit interface. The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those in the archaeal ribosome. The structure also rationalizes much biochemical and genetic data on translation.

Original language	English (US)
Pages (from-to)	1935-1942
Number of pages	8
Journal	Science
Volume	313
Issue number	5795
DOIs	https://doi.org/10.1126/science.1131127
State	Published - Sep 29 2006
Externally published	Yes

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title = "Structure of the 70S ribosome complexed with mRNA and tRNA",

abstract = "The crystal structure of the bacterial 70S ribosome refined to 2.8 angstrom resolution reveals atomic details of its interactions with messenger RNA (mRNA) and transfer RNA (tRNA). A metal ion stabilizes a kink in the mRNA that demarcates the boundary between A and P sites, which is potentially important to prevent slippage of mRNA. Metal ions also stabilize the intersubunit interface. The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those in the archaeal ribosome. The structure also rationalizes much biochemical and genetic data on translation.",

author = "Maria Selmer and Dunham, \{Christine M.\} and \{Murphy IV\}, \{Frank V.\} and Albert Weixlbaumer and Sabine Petry and Kelley, \{Ann C.\} and Weir, \{John R.\} and V. Ramakrishnan",

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AU - Selmer, Maria

AU - Dunham, Christine M.

AU - Murphy IV, Frank V.

AU - Weixlbaumer, Albert

AU - Petry, Sabine

AU - Kelley, Ann C.

AU - Weir, John R.

AU - Ramakrishnan, V.

PY - 2006/9/29

Y1 - 2006/9/29

N2 - The crystal structure of the bacterial 70S ribosome refined to 2.8 angstrom resolution reveals atomic details of its interactions with messenger RNA (mRNA) and transfer RNA (tRNA). A metal ion stabilizes a kink in the mRNA that demarcates the boundary between A and P sites, which is potentially important to prevent slippage of mRNA. Metal ions also stabilize the intersubunit interface. The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those in the archaeal ribosome. The structure also rationalizes much biochemical and genetic data on translation.

AB - The crystal structure of the bacterial 70S ribosome refined to 2.8 angstrom resolution reveals atomic details of its interactions with messenger RNA (mRNA) and transfer RNA (tRNA). A metal ion stabilizes a kink in the mRNA that demarcates the boundary between A and P sites, which is potentially important to prevent slippage of mRNA. Metal ions also stabilize the intersubunit interface. The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those in the archaeal ribosome. The structure also rationalizes much biochemical and genetic data on translation.

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M3 - Article

C2 - 16959973

AN - SCOPUS:33749354360

SN - 0036-8075

VL - 313

SP - 1935

EP - 1942

JO - Science

JF - Science

IS - 5795

ER -

Structure of the 70S ribosome complexed with mRNA and tRNA

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