Structure of the 70S ribosome complexed with mRNA and tRNA

Maria Selmer, Christine M. Dunham, Frank V. Murphy IV, Albert Weixlbaumer, Sabine Petry, Ann C. Kelley, John R. Weir, V. Ramakrishnan

Research output: Contribution to journalArticlepeer-review

1082 Scopus citations


The crystal structure of the bacterial 70S ribosome refined to 2.8 angstrom resolution reveals atomic details of its interactions with messenger RNA (mRNA) and transfer RNA (tRNA). A metal ion stabilizes a kink in the mRNA that demarcates the boundary between A and P sites, which is potentially important to prevent slippage of mRNA. Metal ions also stabilize the intersubunit interface. The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those in the archaeal ribosome. The structure also rationalizes much biochemical and genetic data on translation.

Original languageEnglish (US)
Pages (from-to)1935-1942
Number of pages8
Issue number5795
StatePublished - Sep 29 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General


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