Structure of the α-fetoprotein gene in the mouse

M. B. Gorin; S. M. Tilghman

doi:10.1073/pnas.77.3.1351

Structure of the α-fetoprotein gene in the mouse

M. B. Gorin, S. M. Tilghman

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Abstract

The mouse α-fetoprotein mRNA is the product of a single-copy gene whose mRNA coding sequences are represented discontinuously in the genome. Several EcoRI genomic fragments which contain portions of the α-fetoprotein gene have been cloned by using the EK2 vector λgt WES.λB. In addition, a mouse genomic library has been screened to obtain a 15.75-kilobase segment of DNA that includes more than 85% of the α-fetoprotein coding sequence. Analyses by restriction endonuclease mapping and electron microscopy showed that the mRNA sequence is interrupted by at least 11 intervening sequences which occupy 90% of the cloned DNA.

Original language	English (US)
Pages (from-to)	1351-1355
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	77
Issue number	3 I
DOIs	https://doi.org/10.1073/pnas.77.3.1351
State	Published - 1980

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AB - The mouse α-fetoprotein mRNA is the product of a single-copy gene whose mRNA coding sequences are represented discontinuously in the genome. Several EcoRI genomic fragments which contain portions of the α-fetoprotein gene have been cloned by using the EK2 vector λgt WES.λB. In addition, a mouse genomic library has been screened to obtain a 15.75-kilobase segment of DNA that includes more than 85% of the α-fetoprotein coding sequence. Analyses by restriction endonuclease mapping and electron microscopy showed that the mRNA sequence is interrupted by at least 11 intervening sequences which occupy 90% of the cloned DNA.

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Structure of the α-fetoprotein gene in the mouse

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