Structure of human Nav1.5 reveals the fast inactivation-related segments as a mutational hotspot for the long QT syndrome

Zhangqiang Li, Xueqin Jin, Tong Wu, Xin Zhao, Weipeng Wang, Jianlin Lei, Xiaojing Pan, Nieng Yan

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Nav1.5 is the primary voltage-gated Na+ (Nav) channel in the heart. Mutations of Nav1.5 are associated with various cardiac disorders exemplified by the type 3 long QT syndrome (LQT3) and Brugada syndrome (BrS). E1784K is a common mutation that has been found in both LQT3 and BrS patients. Here we present the cryo-EM structure of the human Nav1.5-E1784K variant at an overall resolution of 3.3 Å. The structure is nearly identical to that of the wild-type human Nav1.5 bound to quinidine. Structural mapping of 91- and 178-point mutations that are respectively associated with LQT3 and BrS reveals a unique distribution pattern for LQT3 mutations. Whereas the BrS mutations spread evenly on the structure, LQT3 mutations are clustered mainly to the segments in repeats III and IV that are involved in gating, voltage-sensing, and particularly inactivation. A mutational hotspot involving the fast inactivation segments is identified and can be mechanistically interpreted by our “door wedge” model for fast inactivation. The structural analysis presented here, with a focus on the impact of mutations on inactivation and late sodium current, establishes a structure-function relationship for the mechanistic understanding of Nav1.5 channelopathies.

Original languageEnglish (US)
Article numbere2100069118
JournalProceedings of the National Academy of Sciences of the United States of America
Volume118
Issue number11
DOIs
StatePublished - 2021

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • Brugada syndrome
  • Fast inactivation
  • Long QT syndrome
  • Na1.5
  • Structure-function relationship

Fingerprint Dive into the research topics of 'Structure of human Na<sub>v</sub>1.5 reveals the fast inactivation-related segments as a mutational hotspot for the long QT syndrome'. Together they form a unique fingerprint.

Cite this