Structure of human NADK2 reveals atypical assembly and regulation of NAD kinases from animal mitochondria

Jin Du, Michael Estrella, Kristina Solorio-Kirpichyan, Philip D. Jeffrey, Alexei Korennykh

Research output: Contribution to journalArticlepeer-review

Abstract

All kingdoms of life produce essential nicotinamide dinucleotide NADP(H) using NAD kinases (NADKs). A panel of published NADK structures from bacteria, eukaryotic cytosol, and yeast mitochondria revealed similar tetrameric enzymes. Here, we present the 2.8-Å structure of the human mitochondrial kinase NADK2 with a bound substrate, which is an exception to this uniformity, diverging both structurally and biochemically from NADKs. We show that NADK2 harbors a unique tetramer disruptor/dimerization element, which is conserved in mitochondrial kinases of animals (EMKA) and absent from other NADKs. EMKA stabilizes the NADK2 dimer but prevents further NADK2 oligomerization by blocking the tetramerization interface. This structural change bears functional consequences and alters the activation mechanism of the enzyme. Whereas tetrameric NADKs undergo cooperative activation via oligomerization, NADK2 is a constitutively active noncooperative dimer. Thus, our data point to a unique regulation of NADP(H) synthesis in animal mitochondria achieved via structural adaptation of the NADK2 kinase.

Original languageEnglish (US)
Article numbere2200923119
JournalProceedings of the National Academy of Sciences of the United States of America
Volume119
Issue number26
DOIs
StatePublished - Jun 28 2022

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • NADK
  • NADK2
  • cooperative
  • dimer
  • structure

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