Structure and developmental expression of the chick α-actin gene

Charles P. Ordahl, Shirley M. Tilghman, Catherine Ovitt, James Fornwald, Michael T. Largen

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Abstract

Recombinant DNA clones containing chick α-actin mRNA sequence have been isolated and used as probes to analyze the structure and developmental expression of the chick α-actin gene. The full length, 2000 nucleotide α-actin mRNA is detected in poly(A) RNA at early and late stages of in vivo leg muscle development. As expected, the α-actin mRNA is present at very low levels at early myogenic stages but is a high abundance species in terminally differentiated muscle. However, most of the α-actin mRNA from fused leg muscle is shorter than 2000 nucleotides, and occurs in relatively discrete size classes. An α-actin-like mRNA can be detected in poly(A) RHA from early embryonic brain, indicating that transcription of the α-actin gene may not be strictly muscle-specific at all stages of development.We have identified at least 3, very short (< 100 base pairs) intervening sequences in the α-actin gene which was isolated from a chick genomic library. The structure of the chick α-actin gene differs, therefore, from the structures of actin genes from yeast and Drosophila, both of which contain a single, relatively long, intervening sequence.

Original languageEnglish (US)
Pages (from-to)4989-5005
Number of pages17
JournalNucleic acids research
Volume8
Issue number21
DOIs
StatePublished - Nov 11 1980

All Science Journal Classification (ASJC) codes

  • Genetics

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    Ordahl, C. P., Tilghman, S. M., Ovitt, C., Fornwald, J., & Largen, M. T. (1980). Structure and developmental expression of the chick α-actin gene. Nucleic acids research, 8(21), 4989-5005. https://doi.org/10.1093/nar/8.21.4989